Norlen Lars
Medical Nobel Institute, Department of Cellular and Molecular Biology (CMB), Karolinska Institute, Stockholm, Sweden.
Eur J Dermatol. 2008 May-Jun;18(3):279-84. doi: 10.1684/ejd.2008.0387. Epub 2008 May 13.
The lack of a molecular understanding, at the nanometer level, of skin biology represents one major obstacle towards the advancement of dermatology. High-resolution cryo-transmission electron microscopy (CEMOVIS) and tomography (CETOVIS) of vitreous skin sections can, however, be used to visualize the native molecular organisation of skin. Micrographs obtained by CEMOVIS show more detail and sometimes differ dramatically from those obtained by conventional methods. Further, molecular resolution (1.5-5 nm) 3D reconstructions of skin can be obtained when CEMOVIS is combined with tomography. In vitreous sections the native organization of skin may be preserved down to atomic resolution. CEMOVIS and CETOVIS are consequently ideally suited for molecular skin research.
在纳米水平上缺乏对皮肤生物学的分子层面理解，是皮肤病学发展的一个主要障碍。然而，玻璃体皮肤切片的高分辨率冷冻透射电子显微镜（CEMOVIS）和断层扫描（CETOVIS）可用于可视化皮肤的天然分子结构。通过CEMOVIS获得的显微照片显示出更多细节，有时与传统方法获得的照片有很大差异。此外，当CEMOVIS与断层扫描相结合时，可以获得皮肤的分子分辨率（1.5 - 5纳米）三维重建。在玻璃体切片中，皮肤的天然结构可能被保留到原子分辨率。因此，CEMOVIS和CETOVIS非常适合用于分子皮肤研究。
