De Francesco Vincenzo, Stinco Giuseppe, Laspina Sebastian, Parlangeli Maria Elena, Mariuzzi Laura, Patrone Pasquale
Institute of Dermatology, Department of Clinical and Experimental Pathology and Medicine, University of Udine, Italy.
Eur J Dermatol. 2008 May-Jun;18(3):292-6. doi: 10.1684/ejd.2008.0391. Epub 2008 May 13.
The present study was aimed at evaluating correlations between clinical response and the histochemical and immunohistochemical changes in vitiligo patches treated with narrow-band UVB phototherapy. Eighteen patients, 13 females and 5 males, aged between 15 and 67 years, affected by vitiligo were recruited and 14 patients completed the study. Before starting the narrow-band UVB treatment three biopsies were carried out on the lesional, perilesional and healthy skin of each patient, excluding the face. After 9 months treatment an additional biopsy was performed on repigmented skin areas adjacent to the lesional biopsy site. For the histochemical evaluation, the Masson-Fontana argentaffin reaction was carried out; for the immunohistochemical study, two antigens were studied: tyrosinase and HMB 45. Clinical improvement was assessed by evaluating the repigmented surfaces, expressed in percentages of the respective vitiligo areas. The results obtained confirm the effectiveness and safety of narrow-band UVB phototherapy for vitiligo, in terms of both good clinical-histological results and absence of important side effects. In addition to attesting the efficacy of narrow-band UVB phototherapy, immunohistochemical tests also appeared to have a potential interest for prognostic purposes.
本研究旨在评估窄谱中波紫外线（NB-UVB）光疗治疗白癜风斑块时临床反应与组织化学及免疫组织化学变化之间的相关性。招募了18例年龄在15至67岁之间的白癜风患者，其中女性13例，男性5例，14例患者完成了研究。在开始窄谱中波紫外线治疗前，对每位患者除面部外的皮损、皮损周边及正常皮肤进行三次活检。治疗9个月后，在皮损活检部位相邻的色素再生皮肤区域进行额外的活检。组织化学评估采用Masson-Fontana嗜银反应；免疫组织化学研究检测两种抗原：酪氨酸酶和HMB 45。通过评估色素再生面积来评估临床改善情况，以各白癜风区域的百分比表示。所得结果证实了窄谱中波紫外线光疗治疗白癜风的有效性和安全性，在临床组织学效果良好且无重要副作用方面均得到体现。免疫组织化学检测除了证明窄谱中波紫外线光疗的疗效外，似乎对预后评估也具有潜在意义。
