Pignolet Béatrice, Boullier Séverine, Gelfi Jacqueline, Bozzetti Marjorie, Russo Pierre, Foulon Eliane, Meyer Gilles, Delverdier Maxence, Foucras Gilles, Bertagnoli Stéphane
Université de Toulouse, ENVT, UMR 1225, F-31076 Toulouse, France.
INRA, UMR 1225, F-31076 Toulouse, France.
J Gen Virol. 2008 Jun;89(Pt 6):1371-1379. doi: 10.1099/vir.0.83595-0.
Myxoma virus (MYXV), a leporide-specific poxvirus, represents an attractive candidate for the generation of safe and non-replicative vaccine vectors for other species. With the aim of developing new recombinant vaccines for ruminants, we evaluated the safety and the immunogenicity of recombinant MYXV in sheep. In vitro studies indicated that ovine primary fibroblasts were not permissive for MYXV and that infection of ovine peripheral blood mononuclear cells occurred at a low rate. Although non-specific activation significantly improved the susceptibility of lymphocytes, MYXV infection remained abortive. Histological and immunohistochemical examination at the inoculation sites revealed the development of an inflammatory process and allowed the detection of sparse infected cells in the dermis. In addition, inoculated sheep developed an antibody response directed against MYXV and the product of the transgene. Overall, these results provide the first line of evidence on the potential of MYXV as a viral vector for ruminants.
黏液瘤病毒（MYXV）是一种兔科特异性痘病毒，是为其他物种开发安全且非复制性疫苗载体的理想候选者。为了开发新型反刍动物重组疫苗，我们评估了重组MYXV在绵羊中的安全性和免疫原性。体外研究表明，绵羊原代成纤维细胞对MYXV不敏感，且绵羊外周血单核细胞的感染率较低。尽管非特异性激活显著提高了淋巴细胞的易感性，但MYXV感染仍为流产型。对接种部位的组织学和免疫组织化学检查显示有炎症过程发生，并能检测到真皮中稀疏的感染细胞。此外，接种疫苗的绵羊产生了针对MYXV和转基因产物的抗体反应。总体而言，这些结果为MYXV作为反刍动物病毒载体的潜力提供了初步证据。
