Liu Y N, Klaus A, Kari B, Stinski M F, Eckhardt J, Gehrz R C
Immunobiology Division, Sutton Immunobiology Research Laboratory, Children's Biomedical Research Institute, St. Paul, Minnesota 55102.
J Virol. 1991 Mar;65(3):1644-8. doi: 10.1128/JVI.65.3.1644-1648.1991.
Host defense against human cytomegalovirus (HCMV) involves both humoral and cell-mediated immunity. In this report, human immune responses to glycoproteins encoded by the HCMV gB homolog gene have been examined by using glycoproteins purified by immunoaffinity from HCMV virions and recombinant proteins expressed by vaccinia viruses containing either the entire gB open reading frame or a C-terminal deletion mutant, gBm165, coding for the N-terminal 513 amino acids of gB. Neutralizing antibodies, helper T cells, and cytotoxic T cells reactive with epitopes on the N-terminal portion of gB were detected in some seropositive individuals, suggesting that this region of gB may be important in eliciting protective immunity during natural infection for some individuals.
宿主对人巨细胞病毒（HCMV）的防御涉及体液免疫和细胞介导的免疫。在本报告中，通过使用从HCMV病毒粒子中通过免疫亲和纯化的糖蛋白以及由含有整个gB开放阅读框或C末端缺失突变体gBm165（编码gB的N末端513个氨基酸）的痘苗病毒表达的重组蛋白，研究了人类对HCMV gB同源基因编码的糖蛋白的免疫反应。在一些血清阳性个体中检测到了与gB N末端部分表位反应的中和抗体、辅助性T细胞和细胞毒性T细胞，这表明gB的该区域在某些个体的自然感染期间引发保护性免疫方面可能很重要。
