Gonczol E, Ianacone J, Ho W Z, Starr S, Meignier B, Plotkin S
Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania.
Vaccine. 1990 Apr;8(2):130-6. doi: 10.1016/0264-410x(90)90135-9.
Three human cytomegalovirus (HCMV) seronegative individuals were immunized with a single dose of HCMV envelope; two individuals developed neutralizing antibodies. Two naturally HCMV seropositive and three HCMV seronegative human volunteers were immunized with a major glycoprotein complex, gA/gB, of HCMV that had been purified by immunoadsorbent column chromatography. After a single injection of the gA/gB preparation, the naturally seropositive individuals developed higher titres of neutralizing antibodies and temporarily higher HCMV-specific lymphocyte proliferation (HCMV-LP) responses in vitro. The seronegative individuals developed neutralizing antibodies after the third injection of gA/gB, which were present only transiently, but showed a rapid reappearance and increase in titre after the fourth injection. At 1 year after the first injection, the neutralizing antibody titres were still comparable with those of the naturally seropositive individuals. HCMV-LP responses to HCMV in the initially seronegative individuals developed after the second or third injection with the gA/gB preparation and remained positive during the 1-year observation period. These results show that the gA/gB protein induces both humoral and cellular immune responses in humans, and might serve as the basis of a subunit vaccine.
三名人类巨细胞病毒（HCMV）血清阴性个体接受了单剂量HCMV包膜免疫；两名个体产生了中和抗体。两名自然HCMV血清阳性和三名HCMV血清阴性的人类志愿者用通过免疫吸附柱色谱法纯化的HCMV主要糖蛋白复合物gA/gB进行免疫。单次注射gA/gB制剂后，自然血清阳性个体产生了更高滴度的中和抗体，并且在体外暂时产生了更高的HCMV特异性淋巴细胞增殖（HCMV-LP）反应。血清阴性个体在第三次注射gA/gB后产生了中和抗体，这些抗体仅短暂存在，但在第四次注射后迅速重新出现且滴度增加。首次注射后1年，中和抗体滴度仍与自然血清阳性个体相当。最初血清阴性个体对HCMV的HCMV-LP反应在第二次或第三次注射gA/gB制剂后出现，并在1年观察期内保持阳性。这些结果表明，gA/gB蛋白可在人类中诱导体液免疫和细胞免疫反应，并可能作为亚单位疫苗的基础。
