用于检测女性泌尿生殖系统衣原体感染的基于聚合酶链反应的快速检测法。

Rapid polymerase chain reaction-based test for the detection of female urogenital chlamydial infections.

作者信息

Wiesenfeld H C, Uhrin M, Dixon B W, Sweet R L

机构信息

Department of Obstetrics, Gynecology, and Reproductive Sciences Magee-Womens Hospital University of Pittsburgh School of Medicine 300 Halket Street, Pittsburgh, PA 15213, USA.

出版信息

Infect Dis Obstet Gynecol. 1994;1(4):182-7. doi: 10.1155/S1064744994000050.

DOI:10.1155/S1064744994000050

PMID:18475342

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2364337/

Abstract

OBJECTIVE

The purpose of this study was to evaluate the Amplicor Chlamydia trachomatis Test (Roche Molecular Systems, Branchburg, NJ), a polymerase chain reaction (PCR)-based technique, as a screening test for the detection of female urogenital C. trachomatis infections, comparing it to an enzyme immunoassay method.

METHODS

Endocervical specimens for PCR and Chlamydiazyme (Abbott Laboratories, North Chicago, IL) analysis were obtained from 328 unselected patients at the outpatient Sexually Transmitted Diseases Clinic at the Allegheny County Health Department, Pittsburgh, PA. In addition, urethral swabs for PCR analysis were obtained from 256 of these patients.

RESULTS

By PCR analysis, the prevalence of urogenital chlamydial infections was 15.6% and that of cervical chlamydial infections was 10.7%. The sensitivity of PCR in the detection of endocervical chlamydial infections was 89.7% and the specificity was 100%. The positive and negative predictive values of PCR were 100% and 99%, respectively. The sensitivity of Chlamydiazyme in the detection of cervical infections was 61.5% and the specificity was 99.7%, with a positive predictive value of 96.0%. Among all patients with urogenital chlamydial infections, concomitant infections in the urethra and cervix occurred in 52.5%, whereas the urthra or cervix was solely infected in 35.0% and 12.5%, respectively.

CONCLUSIONS

This PCR-based technique is a rapid screening tool for the diagnosis of urogenital chlamydial infections and is more sensitive than Chlamydiazyme for endocervical infections in a sexually transmitted disease clinic population.

摘要

目的

本研究旨在评估基于聚合酶链反应（PCR）技术的安普瑞康沙眼衣原体检测法（罗氏分子系统公司，新泽西州布兰奇堡），作为检测女性泌尿生殖道沙眼衣原体感染的筛查试验，并与酶免疫测定法进行比较。

方法

从宾夕法尼亚州匹兹堡阿勒格尼县卫生部门性传播疾病门诊的328例未经挑选的患者中获取宫颈标本，用于PCR和衣原体酶免疫测定法（雅培实验室，伊利诺伊州北芝加哥）分析。此外，从其中256例患者中获取尿道拭子用于PCR分析。

结果

通过PCR分析，泌尿生殖道衣原体感染的患病率为15.6%，宫颈衣原体感染的患病率为10.7%。PCR检测宫颈衣原体感染的敏感性为89.7%，特异性为100%。PCR的阳性预测值和阴性预测值分别为100%和99%。衣原体酶免疫测定法检测宫颈感染的敏感性为61.5%，特异性为99.7%，阳性预测值为96.0%。在所有泌尿生殖道衣原体感染患者中，尿道和宫颈同时感染的发生率为52.5%，而仅尿道或宫颈感染的发生率分别为35.0%和12.5%。