IL-7 和 IL-8 抑制去透明带仓鼠卵精子穿透试验中的配子相互作用。
IL-7 and IL-8 inhibit gamete interaction in the zona-free hamster egg sperm penetration assay.
机构信息
Department of Obstetrics and Gynecology Mount Sinai Medical Center Miami Beach FL USA.
出版信息
Mediators Inflamm. 1992;1(1):67-9. doi: 10.1155/S0962935192000139.
Current thought in reproductive endocrinology suggests hat endometriosis-associated subfertility may be the result of an adverse influence of activated immunocompetent cells on fertilization and embryo development. Inflammatory ediators such as interleukin-1 and tumour necrosis actor have been implicated in the pathophysiology of this process. The purpose of this study was to assess the effect of two recently characterized cytokines, interleukin-7 (IL-7 ) and interleukin-8 (IL-8), on gamete interaction in the perm penetration assay (SPA). Donor sperm were preincubated or 4 h with 0.5, 5, 50, or 500 ng ml(-1) of human ecombinant IL-7 or IL-8. Sperm penetration was determined by an experienced gametologist by the presence of decondensed sperm heads or pronuclei formation. A dose-dependent inhibition of gamete interaction was observed following coincubation with either IL-7 or IL-8. These data offer the possibility that IL-7 and IL-8 may play a role in the pathogenesis of immunocompetent cell-associated subfertility.
目前的生殖内分泌学观点认为，子宫内膜异位症相关的不孕可能是激活的免疫活性细胞对受精和胚胎发育产生不利影响的结果。白细胞介素-1 和肿瘤坏死因子等炎症介质已被牵涉到这一过程的病理生理学中。本研究的目的是评估两种最近被描述的细胞因子，白细胞介素-7（IL-7）和白细胞介素-8（IL-8），对精子穿透试验（SPA）中配子相互作用的影响。供体精子与 0.5、5、50 或 500ng/ml 的人重组 IL-7 或 IL-8 预孵育或孵育 4 小时。通过有经验的配子学家根据去凝聚的精子头部或原核形成来确定精子穿透。与 IL-7 或 IL-8 共孵育后观察到配子相互作用呈剂量依赖性抑制。这些数据提供了这样一种可能性，即 IL-7 和 IL-8 可能在免疫活性细胞相关不孕的发病机制中发挥作用。
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