Hill J A, Haimovici F, Anderson D J
Fearing Research Laboratory, Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115.
J Immunol. 1987 Oct 1;139(7):2250-4.
The effects of activated leukocyte products on embryonic development were assessed by adding mouse and human leukocyte culture supernatants and purified murine and human lymphokines and monokines to mouse embryos in tissue culture. Supernatants from mitogen-stimulated and mixed lymphocyte cultures arrested embryonic development at the two-cell to morula stage. Of a panel of six individual lymphokines and monokines tested for effects in this system, both murine and human forms of the lymphokines colony-stimulating factor, interferon-gamma, and human B cell growth factor significantly arrested embryonic development over a wide concentration range. The monokines, interleukin 1 and tumor necrosis factor, also had significant effects but only at high doses. These results indicate that products of activated lymphocytes and macrophages can have detrimental effects on preimplantation embryos. Early abortion could result from local (intrauterine) production of such embryotoxic factors by activated lymphocytes and macrophages in response to stimulation by microorganisms or reproductive tissue antigens.
通过在组织培养中将小鼠和人类白细胞培养上清液以及纯化的小鼠和人类淋巴因子及单核因子添加到小鼠胚胎中，评估活化白细胞产物对胚胎发育的影响。来自有丝分裂原刺激的和混合淋巴细胞培养物的上清液使胚胎发育在二细胞至桑椹胚阶段停滞。在所测试的一组六种单独的淋巴因子和单核因子中，淋巴因子集落刺激因子、干扰素-γ的小鼠和人类形式以及人类B细胞生长因子在很宽的浓度范围内均显著使胚胎发育停滞。单核因子白细胞介素1和肿瘤坏死因子也有显著作用，但仅在高剂量时。这些结果表明活化淋巴细胞和巨噬细胞的产物可对植入前胚胎产生有害影响。早期流产可能是由活化淋巴细胞和巨噬细胞响应微生物或生殖组织抗原刺激而在局部（子宫内）产生此类胚胎毒性因子所致。
