Kimura Y, Ito Y, Nagata I, Yoshi S, Maeno K
Arch Virol. 1976;51(4):275-83. doi: 10.1007/BF01317931.
Surface antigens of HeLaHVJ cells, a cell line persistently infected with HVJ, were studied by fluorescent antibody staining. After absorption with concentrated HVJ virions and HeLa cells, anti-HeLaHVJ antiserum was able to demonstrate specific surface fluorescence on HeLaHVJ cells, while this serum no longer reacted with original HeLa cells nor with HVJ virions. During cytolytic infection of HeLa cells with HVJ, this specific surface antigen appeared at an early stage of infection prior to the appearance of newly synthesized HVJ viral antigens and moreover appeared in spite of the inhibition of viral protein synthesis. This antigen was detected neither on HeLa cells infected with other myxoviruses except HVJ nor on various other kinds of cells infected with HVJ. The specific surface antigen was still found on the HeLaHVJ cell surface after incubation at 38 degrees C for two days, while HVJ structural antigens on the cell surface no longer could be detected. Mild short-term treatment of HeLa cells with trypsin, neuraminidase from vibrio cholerae, phospholipase-C and hyaluronidase failed to expose specific antigen. The antigen was distinguishable from the Forssman and human blood type antigens. The mechanism of appearance of a new antigen on the surface of HeLaHVJ cells remains unclear.
通过荧光抗体染色研究了持续感染仙台病毒（HVJ）的HeLaHVJ细胞系的表面抗原。用浓缩的仙台病毒颗粒和HeLa细胞吸收后，抗HeLaHVJ抗血清能够在HeLaHVJ细胞上显示出特异性表面荧光，而该血清不再与原始HeLa细胞或仙台病毒颗粒发生反应。在用仙台病毒对HeLa细胞进行溶细胞感染期间，这种特异性表面抗原在感染早期出现，早于新合成的仙台病毒抗原出现，而且尽管病毒蛋白合成受到抑制仍会出现。在感染除仙台病毒之外的其他黏液病毒的HeLa细胞上，以及在感染仙台病毒的各种其他类型细胞上均未检测到这种抗原。在38℃孵育两天后，仍可在HeLaHVJ细胞表面发现特异性表面抗原，而细胞表面的仙台病毒结构抗原则不再能被检测到。用胰蛋白酶、霍乱弧菌神经氨酸酶、磷脂酶C和透明质酸酶对HeLa细胞进行轻度短期处理未能暴露特异性抗原。该抗原与福斯曼抗原和人类血型抗原不同。HeLaHVJ细胞表面新抗原出现的机制尚不清楚。
