Kimura Y, Yokochi T, Miyadai T, Yoshida K, Yokoo J, Matsumoto K
J Virol. 1985 Mar;53(3):980-3. doi: 10.1128/JVI.53.3.980-983.1985.
A mutant cell line of porcine kidney cells that resists the cytopathic effect of influenza virus has been obtained and characterized. These cells, designated ESK-R, were originally obtained by prolonged cultivation of cells surviving influenza B/Kanagawa/73 virus infection. No infectious virus was recovered from ESK-R cells, and no evidence for the presence of virus antigens in the cells was demonstrated by immunofluorescent staining. ESK-R cells also showed a distinct resistance to various other strains of both types A and B influenza viruses. The growth of mumps, Sendai, or Newcastle disease virus was considerably restricted, but the cell line normally supported the replication of vesicular stomatitis virus. ESK-R cells were found to lack specific receptors for influenza virus as determined by fluorescence-activated cell sorter analyses. The membrane barrier of ESK-R cells was successfully overcome by nonspecific endocytosis of calcium-coprecipitated virus particles followed by production of an appreciable amount of progeny virus.
已获得并鉴定出一种能抵抗流感病毒细胞病变效应的猪肾细胞突变株系。这些细胞被命名为ESK-R，最初是通过对在乙型流感病毒/神奈川/73感染中存活的细胞进行长期培养而获得的。从ESK-R细胞中未回收感染性病毒，并且免疫荧光染色未显示细胞中存在病毒抗原的证据。ESK-R细胞对A、B两型流感病毒的各种其他毒株也表现出明显的抗性。腮腺炎、仙台或新城疫病毒的生长受到很大限制，但该细胞株系通常支持水疱性口炎病毒的复制。通过荧光激活细胞分选分析确定，ESK-R细胞缺乏流感病毒的特异性受体。通过钙共沉淀病毒颗粒的非特异性内吞作用，随后产生大量子代病毒，成功克服了ESK-R细胞的膜屏障。
