缺乏磷脂酰肌醇特异性磷脂酶C的单核细胞增生李斯特菌突变体无致病性。
Listeria monocytogenes mutants lacking phosphatidylinositol-specific phospholipase C are avirulent.
作者信息
Camilli A, Goldfine H, Portnoy D A
机构信息
Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia 19104.
出版信息
J Exp Med. 1991 Mar 1;173(3):751-4. doi: 10.1084/jem.173.3.751.
Abstract
A number of bacterial species secrete phosphatidylinositol-specific phospholipase C (PI-PLC). In this report, we show that the facultative intracellular bacterial pathogen, Listeria monocytogenes, contains a gene, plcA, predicting a polypeptide with 31% amino acid identity to a Bacillus thuringiensis PI-PLC. Accordingly, L. monocytogenes secretes PI-PLC activity, while a mutant with a transposon insertion in plcA lacks detectable PI-PLC activity. In addition, expression of plcA in B. subtilis resulted in secretion of PI-PLC activity. The L. monocytogenes PI-PLC-defective mutant was three logs less virulent for mice and failed to grow in host tissues. The mutant was also defective for in vitro growth in mouse peritoneal macrophages. These results strongly suggest that PI-PLC is an essential determinant of L. monocytogenes pathogenesis. Whether the PI-PLC acts on a bacterial or host substrate remains to be determined.
摘要
许多细菌物种会分泌磷脂酰肌醇特异性磷脂酶C（PI-PLC）。在本报告中，我们表明兼性胞内细菌病原体单核细胞增生李斯特菌含有一个基因plcA，预测其编码的多肽与苏云金芽孢杆菌PI-PLC有31%的氨基酸同一性。相应地，单核细胞增生李斯特菌分泌PI-PLC活性，而在plcA中插入转座子的突变体缺乏可检测到的PI-PLC活性。此外，plcA在枯草芽孢杆菌中的表达导致PI-PLC活性的分泌。单核细胞增生李斯特菌PI-PLC缺陷型突变体对小鼠的毒力降低了三个对数级，并且无法在宿主组织中生长。该突变体在小鼠腹腔巨噬细胞中的体外生长也存在缺陷。这些结果强烈表明PI-PLC是单核细胞增生李斯特菌致病机制的一个关键决定因素。PI-PLC作用于细菌还是宿主底物仍有待确定。
相似文献
1
Listeria monocytogenes mutants lacking phosphatidylinositol-specific phospholipase C are avirulent.缺乏磷脂酰肌醇特异性磷脂酶C的单核细胞增生李斯特菌突变体无致病性。
J Exp Med. 1991 Mar 1;173(3):751-4. doi: 10.1084/jem.173.3.751.
2
Identification of phosphatidylinositol-specific phospholipase C activity in Listeria monocytogenes: a novel type of virulence factor?单核细胞增生李斯特菌中磷脂酰肌醇特异性磷脂酶C活性的鉴定：一种新型毒力因子？
Mol Microbiol. 1991 Feb;5(2):367-72. doi: 10.1111/j.1365-2958.1991.tb02118.x.
3
Dual roles of plcA in Listeria monocytogenes pathogenesis.plcA在单核细胞增生李斯特菌致病机制中的双重作用。
Mol Microbiol. 1993 Apr;8(1):143-57. doi: 10.1111/j.1365-2958.1993.tb01211.x.
4
Phosphatidylinositol-specific phospholipase C from Listeria monocytogenes contributes to intracellular survival and growth of Listeria innocua.来自单核细胞增生李斯特菌的磷脂酰肌醇特异性磷脂酶C有助于无害李斯特菌在细胞内的存活和生长。
Infect Immun. 1994 Nov;62(11):4795-803. doi: 10.1128/iai.62.11.4795-4803.1994.
5
Characterization of Listeria monocytogenes expressing anthrolysin O and phosphatidylinositol-specific phospholipase C from Bacillus anthracis.表达炭疽芽孢杆菌溶血素O和磷脂酰肌醇特异性磷脂酶C的单核细胞增生李斯特菌的特性分析
Infect Immun. 2005 Oct;73(10):6639-46. doi: 10.1128/IAI.73.10.6639-6646.2005.
6
Listeria monocytogenes phosphatidylinositol-specific phospholipase C has evolved for virulence by greatly reduced activity on GPI anchors.单核细胞增生李斯特菌磷脂酰肌醇特异性磷脂酶C通过对糖基磷脂酰肌醇（GPI）锚定物活性的大幅降低而进化出毒力。
Proc Natl Acad Sci U S A. 2005 Sep 6;102(36):12927-31. doi: 10.1073/pnas.0501725102. Epub 2005 Aug 23.
7
8
Mutagenesis of active-site histidines of Listeria monocytogenes phosphatidylinositol-specific phospholipase C: effects on enzyme activity and biological function.单核细胞增生李斯特菌磷脂酰肌醇特异性磷脂酶C活性位点组氨酸的诱变：对酶活性和生物学功能的影响。
Infect Immun. 1999 Jan;67(1):182-6. doi: 10.1128/IAI.67.1.182-186.1999.
9
[Cloning and expression of the phosphatidylinositol-specific phospholipase C gene from Listeria monocytogenes].[单核细胞增生李斯特菌磷脂酰肌醇特异性磷脂酶C基因的克隆与表达]
Mol Gen Mikrobiol Virusol. 1994 Nov-Dec(6):3-8.
10
Correlation between the presence of virulence-associated genes as determined by PCR and actual virulence to mice in various strains of Listeria spp.通过聚合酶链反应（PCR）测定的毒力相关基因的存在与不同李斯特菌菌株对小鼠的实际毒力之间的相关性
Microbiol Immunol. 1995;39(5):343-9. doi: 10.1111/j.1348-0421.1995.tb02211.x.
引用本文的文献
1
Pathogenesis: The Role of Stress Adaptation.发病机制：应激适应的作用
Microorganisms. 2022 Jul 27;10(8):1522. doi: 10.3390/microorganisms10081522.
2
Virulence Mechanisms of : Current Knowledge and Implications for Vaccine Design.《：当前认知及对疫苗设计的意义》的毒力机制
Front Microbiol. 2022 Mar 3;13:842017. doi: 10.3389/fmicb.2022.842017. eCollection 2022.
3
NAD(H)-mediated tetramerization controls the activity of phospholipase PlaB.NAD(H)-介导的四聚化控制磷脂酶 PlaB 的活性。
Proc Natl Acad Sci U S A. 2021 Jun 8;118(23). doi: 10.1073/pnas.2017046118.
4
Phosphatidylinositol-specific phospholipase C enhances epidermal penetration by Staphylococcus aureus.磷脂酰肌醇特异性磷脂酶 C 增强金黄色葡萄球菌的表皮穿透性。
Sci Rep. 2020 Oct 20;10(1):17845. doi: 10.1038/s41598-020-74692-8.
5
virulence factors, including listeriolysin O, are secreted in biologically active extracellular vesicles.毒力因子，包括李斯特菌溶血素 O，被分泌到具有生物活性的细胞外囊泡中。
J Biol Chem. 2019 Jan 25;294(4):1202-1217. doi: 10.1074/jbc.RA118.006472. Epub 2018 Nov 30.
6
Identification and evaluation of the novel immunodominant antigen Rv2351c from Mycobacterium tuberculosis.结核分枝杆菌新型免疫显性抗原Rv2351c的鉴定与评价
Emerg Microbes Infect. 2017 Jun 7;6(6):e48. doi: 10.1038/emi.2017.34.
7
A Fluorescent Probe for Detecting and Identifying Genes Critical for Cell Entry.一种用于检测和鉴定细胞进入关键基因的荧光探针。
Front Microbiol. 2016 Dec 20;7:2021. doi: 10.3389/fmicb.2016.02021. eCollection 2016.
8
Listeria monocytogenes and the Inflammasome: From Cytosolic Bacteriolysis to Tumor Immunotherapy.单核细胞增生李斯特菌与炎性小体：从胞质细菌溶解到肿瘤免疫治疗
Curr Top Microbiol Immunol. 2016;397:133-60. doi: 10.1007/978-3-319-41171-2_7.
9
Bacterial Sphingomyelinases and Phospholipases as Virulence Factors.作为毒力因子的细菌鞘磷脂酶和磷脂酶
Microbiol Mol Biol Rev. 2016 Jun 15;80(3):597-628. doi: 10.1128/MMBR.00082-15. Print 2016 Sep.
10
Recombinant broad-range phospholipase C from Listeria monocytogenes exhibits optimal activity at acidic pH.来自单核细胞增生李斯特菌的重组广谱磷脂酶C在酸性pH值下表现出最佳活性。
Biochim Biophys Acta. 2016 Jun;1864(6):697-705. doi: 10.1016/j.bbapap.2016.03.008. Epub 2016 Mar 11.
本文引用的文献
1
Phosphatidylinositol-specific phospholipase C from Staphylococcus aureus.来自金黄色葡萄球菌的磷脂酰肌醇特异性磷脂酶C
Methods Enzymol. 1981;71 Pt C:741-6. doi: 10.1016/0076-6879(81)71087-5.
2
Lithium amplifies agonist-dependent phosphatidylinositol responses in brain and salivary glands.锂可增强大脑和唾液腺中激动剂依赖性磷脂酰肌醇反应。
Biochem J. 1982 Sep 15;206(3):587-95. doi: 10.1042/bj2060587.
3
Phospholipids of Listeria monocytogenes.单核细胞增生李斯特菌的磷脂
Biochim Biophys Acta. 1971 Sep 1;239(3):428-42. doi: 10.1016/0005-2760(71)90035-x.
4
Sequence of the Bacillus thuringiensis phosphatidylinositol specific phospholipase C.苏云金芽孢杆菌磷脂酰肌醇特异性磷脂酶C的序列
Nucleic Acids Res. 1988 Nov 11;16(21):10383. doi: 10.1093/nar/16.21.10383.
5
In vitro model of penetration and intracellular growth of Listeria monocytogenes in the human enterocyte-like cell line Caco-2.单核细胞增生李斯特菌在人肠上皮样细胞系Caco-2中的穿透及细胞内生长的体外模型
Infect Immun. 1987 Nov;55(11):2822-9. doi: 10.1128/iai.55.11.2822-2829.1987.
6
Molecular Koch's postulates applied to microbial pathogenicity.应用于微生物致病性的分子科赫法则。
Rev Infect Dis. 1988 Jul-Aug;10 Suppl 2:S274-6. doi: 10.1093/cid/10.supplement_2.s274.
7
Rapid and sensitive protein similarity searches.快速且灵敏的蛋白质相似性搜索。
Science. 1985 Mar 22;227(4693):1435-41. doi: 10.1126/science.2983426.
8
Role of hemolysin for the intracellular growth of Listeria monocytogenes.溶血素在单核细胞增生李斯特菌细胞内生长中的作用。
J Exp Med. 1988 Apr 1;167(4):1459-71. doi: 10.1084/jem.167.4.1459.
9
The glycosyl-phosphatidylinositol anchor of membrane proteins.膜蛋白的糖基磷脂酰肌醇锚定物。
Biochim Biophys Acta. 1989 Dec 6;988(3):427-54. doi: 10.1016/0304-4157(89)90014-2.
10
Gamma interferon limits access of Listeria monocytogenes to the macrophage cytoplasm.γ干扰素限制单核细胞增生李斯特菌进入巨噬细胞胞质。
J Exp Med. 1989 Dec 1;170(6):2141-6. doi: 10.1084/jem.170.6.2141.
Zotero 插件