Pechanova O, Stone W D, Monroe W, Nebeker T E, Klepzig K D, Yuceer C
Department of Forestry, Mississippi State University, Mississippi State, MS 39762, USA.
Insect Mol Biol. 2008 Jun;17(3):261-77. doi: 10.1111/j.1365-2583.2008.00801.x.
The southern pine beetle (Dendroctonus frontalis Zimmermann) kills all pines within its range and is among the most important forest pest species in the US. Using a specialized mycangium surrounded by gland cells in the pronotum, adult females culture, transport, and inoculate two fungi into beetle galleries during oviposition. These fungal symbionts, to varying degrees, exclude antagonistic fungi and provide nutrients to larvae. However, the mechanisms (e.g. secreted antibiotic chemicals or nutrients, proteins or pathways) by which this relationship is maintained are not known. Here we present the first global and differential proteome profile of the southern pine beetle pronotum. Two-dimensional polyacrylamide electrophoresis, tandem mass spectrometry, and database searches revealed that the majority of pronotal proteins were related to energy-yielding metabolism, contractile apparati, cell structure, and defence. The identified proteins provide important insights into the molecular and biochemical processes of, and candidates for functional genomics to understand mycangia and pronotum functions in, the southern pine beetle.
南方松甲虫（Dendroctonus frontalis Zimmermann）会杀死其活动范围内的所有松树，是美国最重要的森林害虫物种之一。成年雌性南方松甲虫利用前胸背板中被腺细胞包围的特殊菌囊，在产卵期间培养、运输两种真菌并将其接种到虫道中。这些真菌共生体在不同程度上排斥拮抗性真菌，并为幼虫提供营养。然而，维持这种关系的机制（例如分泌的抗生素化学物质或营养物质、蛋白质或途径）尚不清楚。在此，我们展示了南方松甲虫前胸背板的首个全蛋白质组和差异蛋白质组图谱。二维聚丙烯酰胺凝胶电泳、串联质谱分析和数据库搜索显示，前胸背板的大多数蛋白质与能量代谢、收缩装置、细胞结构和防御有关。所鉴定的蛋白质为了解南方松甲虫菌囊和前胸背板功能的分子和生化过程以及功能基因组学的候选物提供了重要见解。
