Ecosystem Science and Management Program, University of Northern British Columbia, 3333 University Way, Prince George, BC, Canada V2N 4Z9.
Insect Biochem Mol Biol. 2012 Dec;42(12):890-901. doi: 10.1016/j.ibmb.2012.08.003. Epub 2012 Sep 12.
Mountain pine beetles, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae), are native to western North America, but have recently begun to expand their range across the Canadian Rocky Mountains. The requirement for larvae to withstand extremely cold winter temperatures and potentially toxic host secondary metabolites in the midst of their ongoing development makes this a critical period of their lives.
We have uncovered global protein profiles for overwintering mountain pine beetle larvae. We have also quantitatively compared the proteomes for overwintering larvae sampled during autumn cooling and spring warming using iTRAQ methods. We identified 1507 unique proteins across all samples. In total, 33 proteins exhibited differential expression (FDR < 0.05) when compared between larvae before and after a cold snap in the autumn; and 473 proteins exhibited differential expression in the spring when measured before and after a steady incline in mean daily temperature. Eighteen proteins showed significant changes in both autumn and spring samples.
These first proteomic data for mountain pine beetle larvae show evidence of the involvement of trehalose, 2-deoxyglucose, and antioxidant enzymes in overwintering physiology; confirm and expand upon previous work implicating glycerol in cold tolerance in this insect; and provide new, detailed information on developmental processes in beetles. These results and associated data will be an invaluable resource for future targeted research on cold tolerance mechanisms in the mountain pine beetle and developmental biology in coleopterans.
落矶山松甲虫（Dendroctonus ponderosae Hopkins）原产于北美洲西部，但最近已开始跨越加拿大落矶山脉扩展其分布范围。幼虫需要在冬季极端寒冷的温度和持续发育过程中潜在的有毒宿主次生代谢物中生存，这是它们生命中的关键时期。
我们已经揭示了越冬季落矶山松甲虫幼虫的全球蛋白质图谱。我们还使用 iTRAQ 方法定量比较了秋季降温期间和春季升温期间采集的越冬季幼虫的蛋白质组。我们在所有样本中鉴定了 1507 种独特的蛋白质。总共有 33 种蛋白质在秋季寒冷时期幼虫前后的表达存在差异（FDR<0.05）；在春季，当测量平均日温度稳定上升前后时，有 473 种蛋白质的表达存在差异。18 种蛋白质在秋季和春季样本中均显示出显著变化。
这些落矶山松甲虫幼虫的首批蛋白质组数据表明，海藻糖、2-脱氧葡萄糖和抗氧化酶参与了越冬生理学；证实并扩展了先前关于甘油在这种昆虫耐寒性中的作用的工作；并提供了有关甲虫发育过程的新的详细信息。这些结果和相关数据将成为未来针对落矶山松甲虫耐寒机制和鞘翅目动物发育生物学的目标研究的宝贵资源。
