使用固定化脂肪酶通过大豆油的酯交换反应生产生物柴油燃料。
Biodiesel fuel production by the transesterification reaction of soybean oil using immobilized lipase.
作者信息
Bernardes Otávio L, Bevilaqua Juliana V, Leal Márcia C M R, Freire Denise M G, Langone Marta A P
机构信息
Instituto de Química, Universidade do Estado do Rio de Janeiro, Rua São Francisco Xavier, 524, PHLC, sl. 427, CEP: 20559-900, RJ, RJ, Brazil.
出版信息
Appl Biochem Biotechnol. 2007 Apr;137-140(1-12):105-14. doi: 10.1007/s12010-007-9043-5.
The enzymatic alcoholysis of soybean oil with methanol and ethanol was investigated using a commercial, immobilized lipase (Lipozyme RMIM). The effect of alcohol (methanol or ethanol), enzyme concentration, molar ratio of alcohol to soybean oil, solvent, and temperature on biodiesel production was determined. The best conditions were obtained in a solvent-free system with ethanol/oil molar ratio of 3.0, temperature of 50 degrees C, and enzyme concentration of 7.0% (w/w). Three-step batch ethanolysis was most effective for the production of biodiesel. Ethyl esters yield was about 60% after 4 h of reaction.
使用市售固定化脂肪酶（Lipozyme RMIM）研究了大豆油与甲醇和乙醇的酶促醇解反应。测定了醇（甲醇或乙醇）、酶浓度、醇与大豆油的摩尔比、溶剂和温度对生物柴油生产的影响。在无溶剂体系中，乙醇/油摩尔比为3.0、温度为50℃、酶浓度为7.0%（w/w）时获得了最佳条件。三步间歇乙醇解反应对生物柴油的生产最为有效。反应4小时后，乙酯产率约为60%。
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