Van Walsum G Peter, Garcia-Gil Maurilio, Chen Shou-Feng, Chambliss Kevin
Department of Environmental Studies, Baylor University, One Bear Place No. 97266, Waco, Texas 76798-7266, USA.
Appl Biochem Biotechnol. 2007 Apr;137-140(1-12):301-11. doi: 10.1007/s12010-007-9060-4.
Liquid hot water pretreatment has been proposed as a possible means of improving rates of enzymatic hydrolysis of biomass while maintaining low levels of inhibitory compounds. Supplementation of liquid hot water pretreatment with dissolved carbon dioxide, yielding carbonic acid, has been shown to improve hydrolysis of some biomass substrates compared with the use of water alone. Previous studies on the application of carbonic acid to biomass pretreatment have noted a higher pH of hydrolyzates treated with carbonic acid as compared with the samples prepared with water alone. This study has applied recently developed analytical methods to quantify the concentration of organic acids in liquid hot water pretreated hydrolyzates, prepared with and without the addition of carbonic acid. It was observed that the addition of carbon dioxide to liquid hot water pretreatment significantly changed the accumulated concentrations of most measured compounds. However, the measured differences in product concentrations resulting from addition of carbonic acid did not account for the measured differences in hydrolyzate pH.
液态热水预处理已被提议作为一种在保持低水平抑制性化合物的同时提高生物质酶水解速率的可能方法。与单独使用水相比，在液态热水预处理中添加溶解的二氧化碳以生成碳酸，已被证明可以改善某些生物质底物的水解。先前关于碳酸在生物质预处理中的应用研究指出，与仅用水制备的样品相比，用碳酸处理的水解产物的pH值更高。本研究应用了最近开发的分析方法来量化在添加和不添加碳酸的情况下液态热水预处理水解产物中有机酸的浓度。观察到在液态热水预处理中添加二氧化碳显著改变了大多数被测化合物的累积浓度。然而，添加碳酸导致的产物浓度测量差异并不能解释水解产物pH值的测量差异。
