Flick M B, Kennedy A R
Department of Radiation Oncology, University of Pennsylvania School of Medicine, Philadelphia 19104.
Cancer Lett. 1991 Feb;56(2):103-8. doi: 10.1016/0304-3835(91)90083-t.
We have examined the effect of protease inhibitors on radiation-induced DNA amplification in vitro using an SV40-transformed Chinese hamster embryo cell line. DNA from cells irradiated with X-rays (10 Gy) or ultraviolet radiation (8 J/m2) resulted in an 8-fold or greater amplification of SV40 sequences compared with unirradiated cells. Addition of antipain or the soybean-derived Bowman-Birk protease inhibitor (BBI) to the culture medium, either 75 min after X-irradiation or within 15 min after ultraviolet irradiation, resulted in a reduction of amplification levels. BBI was more effective than antipain at an equal concentration. These results suggest that a protease may be involved in DNA amplification.
我们使用一种经SV40转化的中国仓鼠胚胎细胞系，在体外研究了蛋白酶抑制剂对辐射诱导的DNA扩增的影响。与未受辐射的细胞相比，用X射线（10 Gy）或紫外线（8 J/m2）照射的细胞的DNA导致SV40序列扩增了8倍或更多。在X射线照射后75分钟或紫外线照射后15分钟内，向培养基中添加抗蛋白酶或大豆衍生的鲍曼-伯克蛋白酶抑制剂（BBI），导致扩增水平降低。在同等浓度下，BBI比抗蛋白酶更有效。这些结果表明蛋白酶可能参与了DNA扩增。
