de Lima C J B, França F P, Sérvulo E F C, Resende M M, Cardoso V L
Universidade Federal de Uberlândia/FEQ, Caixa Postal 593, 38400-902, Uberlândia, MG, Brazil.
Appl Biochem Biotechnol. 2007 Apr;137-140(1-12):463-70. doi: 10.1007/s12010-007-9072-0.
In the present work, the production of rhamnolipid from residual soybean oil (RSO) from food frying facilities was studied using a strain of Pseudomonas aeruginosa of contaminated lagoon, isolated from a hydrocarbon contaminated soil. The optimization of RSO, ammonium nitrate, and brewery residual yeast concentrations was accomplished by a central composite experimental design and surface response analysis. The experiments were performed in 500-mL Erlenmeyer flasks containing 50 mL of mineral medium, at 170 rpm and 30 +/- 1 degrees C, for a 48-h fermentation period. Rhamnolipid production has been monitored by measurements of surface tension, rhamnose concentration, and emulsifying activity. The best-planned results, located on the central point, have corresponded to 22 g/L of RSO, 5.625 g/L of NH(4)NO(3), and 11.5 g/L of brewery yeast. At the maximum point the values for rhamnose and emulsifying index were 2.2 g/L and 100%, respectively.
在本研究中，使用从受烃污染土壤中分离出的受污染泻湖中的一株铜绿假单胞菌，研究了利用食品油炸设施产生的残留大豆油（RSO）生产鼠李糖脂的情况。通过中心复合实验设计和表面响应分析，对RSO、硝酸铵和啤酒厂残留酵母浓度进行了优化。实验在装有50 mL矿物培养基的500 mL锥形瓶中进行，转速为170 rpm，温度为30±1℃，发酵周期为48小时。通过测量表面张力、鼠李糖浓度和乳化活性来监测鼠李糖脂的产生。位于中心点的最佳规划结果对应于22 g/L的RSO、5.625 g/L的NH₄NO₃和11.5 g/L的啤酒厂酵母。在最大值点，鼠李糖和乳化指数的值分别为2.2 g/L和100%。
