Nitschke Marcia, Costa Siddhartha G V A O, Haddad Renato, Gonçalves Lireny A G, Eberlin Marcos N, Contiero Jonas
Department of Biochemistry and Microbiology, Institute of Biological Sciences, São Paulo State University, UNESP/ Rio Claro, Av. 24-A, 1515 Bela Vista CEP 13506-900, Caixa Postal 199, Rio Claro SP, Brazil.
Biotechnol Prog. 2005 Sep-Oct;21(5):1562-6. doi: 10.1021/bp050198x.
Oil wastes were evaluated as alternative low-cost substrates for the production of rhamnolipids by Pseudomonas aeruginosa LBI strain. Wastes obtained from soybean, cottonseed, babassu, palm, and corn oil refinery were tested. The soybean soapstock waste was the best substrate, generating 11.7 g/L of rhamnolipids with a surface tension of 26.9 mN/m, a critical micelle concentration of 51.5 mg/L, and a production yield of 75%. The monorhamnolipid RhaC(10)C(10) predominates when P. aeruginosa LBI was cultivated on hydrophobic substrates, whereas hydrophilic carbon sources form the dirhamnolipid Rha(2)C(10)C(10) predominantly.
对油类废弃物作为铜绿假单胞菌LBI菌株生产鼠李糖脂的低成本替代底物进行了评估。测试了从大豆、棉籽、巴巴苏、棕榈和玉米油精炼厂获得的废弃物。大豆皂脚废弃物是最佳底物，能产生11.7 g/L的鼠李糖脂，表面张力为26.9 mN/m，临界胶束浓度为51.5 mg/L，产率为75%。当铜绿假单胞菌LBI在疏水底物上培养时，单鼠李糖脂RhaC(10)C(10)占主导，而亲水性碳源主要形成双鼠李糖脂Rha(2)C(10)C(10)。
