[克罗恩病患者血清蛋白质组变异研究]
[Serum proteomic variation study in patients with Crohn disease].
作者信息
Kang Liang, Yang Zu-Li, Liu Wei, Zhang Long-Juan, Liu Shao-Jun, Huang Mei-Jin, Li Ming-Tao, Wang Jian-Ping
机构信息
Department of Colorectal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou 510655, China.
出版信息
Zhonghua Wei Chang Wai Ke Za Zhi. 2008 May;11(3):266-9.
OBJECTIVE
To search differentially expressed proteins in serum of patients with Crohn disease.
METHODS
Serum protein samples from 4 patients with Crohn disease and 8 healthy adults were recruited cross-labeled with variant CyDye, and then followed by two-dimensional differential in-gel electrophoresis (2-D DIGE), image analysis, and identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS).
RESULTS
The 2-D electrophoresis results were compared between the Crohn disease patients and the healthy adults. The spot 1058 expression in serum of Crohn disease patients increased by 1.68 folds as compared with healthy adults (P<0.05). The protein was identified as haptoglobin by mass spectrometry.
CONCLUSION
Up-regulating expression of haptoglobin in serum of Crohn disease patients may play a role in disequilibrium of immunity system.
目的
寻找克罗恩病患者血清中差异表达的蛋白质。
方法
招募4例克罗恩病患者和8名健康成年人的血清蛋白样本，用不同的CyDye进行交叉标记，然后进行二维差异凝胶电泳（2-D DIGE）、图像分析，并通过基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS）进行鉴定。
结果
比较了克罗恩病患者和健康成年人的二维电泳结果。与健康成年人相比，克罗恩病患者血清中斑点1058的表达增加了1.68倍（P<0.05）。经质谱鉴定该蛋白质为触珠蛋白。
结论
克罗恩病患者血清中触珠蛋白表达上调可能在免疫系统失衡中起作用。
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