Cao Jie, Tang Minghua, Li Wanglin L, Xie Jintang, Du Hong, Tang Weibiao B, Wang Hui, Chen Xiwen W, Xiao Huanqing, Li Yuyuan
Department of Gastrointestinal Surgery, Affiliated Guangzhou First Municipal People's Hospital, Guangzhou Medical College, Guangzhou, China.
Int J Surg Pathol. 2009 Feb;17(1):16-21. doi: 10.1177/1066896908315813. Epub 2008 May 14.
The aim of this study is to investigate the expression of AP-4, VEGF, and MMP-9 genes in human colorectal cancer. The expression pattern of activator protein-4 in 160 colorectal cancer compared with 32 colorectal adenomas and 32 normal colorectal tissues is demonstrated by tissue microarray-immunohistochemistry and real-time reverse transcriptase-polymerase chain reaction. Apoptosis status using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling by comparing activator protein-4 positive versus activator protein-4 negative colorectal cancer is also assessed. The messenger RNA levels of vascular endothelial growth factor and matrix metalloproteinase-9 expression in activator protein-4 positive and negative colorectal cancer were measured using real-time reverse transcriptase- polymerase chain reaction. The activator protein-4 expression in normal colorectal tissue, adenoma, and adenocarcinoma were 4 of 32, 8 of 32, and 78 of 160, respectively. It is shown that the activator protein-4 expression was significantly correlated with the progression of colorectal cancer (P < .01) and differentiation and lymph node metastasis (P < .01). Our results also presented that the activator protein-4 expression was associated with the expression of matrix metalloproteinase-9 and vascular endothelial growth factor in the advanced colorectal cancer.
本研究旨在探讨AP - 4、VEGF和MMP - 9基因在人大肠癌中的表达情况。通过组织芯片免疫组化和实时逆转录聚合酶链反应，展示了160例大肠癌中激活蛋白-4的表达模式，并与32例大肠腺瘤和32例正常大肠组织进行比较。还通过比较激活蛋白-4阳性与激活蛋白-4阴性的大肠癌，利用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法评估细胞凋亡状态。采用实时逆转录聚合酶链反应测量激活蛋白-4阳性和阴性大肠癌中血管内皮生长因子和基质金属蛋白酶-9表达的信使RNA水平。正常大肠组织、腺瘤和腺癌中激活蛋白-4的表达分别为32例中的4例、32例中的8例和160例中的78例。结果表明，激活蛋白-4的表达与大肠癌的进展显著相关（P <.01），与分化和淋巴结转移也显著相关（P <.01）。我们的结果还表明，在进展期大肠癌中，激活蛋白-4的表达与基质金属蛋白酶-9和血管内皮生长因子的表达相关。
