Khot Anna C, Bingham Georgina, Field Linda M, Moores Graham D
Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK.
Pest Manag Sci. 2008 Nov;64(11):1139-42. doi: 10.1002/ps.1603.
Conventional in vitro assays sometimes fail to reveal esterase inhibition by piperonyl butoxide (PBO), although synergism studies suggest loss of esterase-mediated sequestration of insecticide does take place. A new in vitro assay has been devised that routinely reveals binding between PBO and these esterases.
The new 'esterase interference' assay detects the blockade of resistance-associated esterases in a species, Myzus persicae Sülzer, where this has not previously been seen. The assay also demonstrates directly the protective effect esterases may confer to target sites of insecticides.
The new assay reveals esterase blockade by PBO and thus has the potential to be used as a high-throughput screening method for other potential synergists.
传统的体外试验有时无法揭示胡椒基丁醚（PBO）对酯酶的抑制作用，尽管增效研究表明酯酶介导的杀虫剂隔离作用确实会丧失。一种新的体外试验已经设计出来，它能够常规地揭示PBO与这些酯酶之间的结合。
新的“酯酶干扰”试验检测到了在烟蚜（Myzus persicae Sülzer）中抗性相关酯酶的阻断情况，而此前在该物种中尚未观察到这种情况。该试验还直接证明了酯酶可能赋予杀虫剂靶位点的保护作用。
新试验揭示了PBO对酯酶的阻断作用，因此有潜力用作其他潜在增效剂的高通量筛选方法。
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