西非贝宁南部埃及伊蚊和白纹伊蚊的抗杀虫剂状况

Insecticide resistance status of Aedes aegypti and Aedes albopictus mosquitoes in southern Benin, West Africa.

作者信息

Konkon Alphonse Keller, Padonou Germain Gil, Osse Razaki, Salako Albert Sourou, Zoungbédji David Mahouton, Sina Haziz, Sovi Arthur, Tokponnon Filemon, Aïkpon Rock, Noukpo Herbert, Baba-Moussa Lamine, Akogbéto Martin Codjo

机构信息

Centre de Recherche Entomologique de Cotonou (CREC), 06 BP 2604, Cotonou, Benin.

Faculté des Sciences et Techniques de l'Université d'Abomey-Calavi, Abomey-Calavi, Benin.

出版信息

Trop Med Health. 2023 Apr 21;51(1):22. doi: 10.1186/s41182-023-00514-y.

DOI:10.1186/s41182-023-00514-y

PMID:37085936

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10122308/

Abstract

BACKGROUND

The emergence of insecticide resistance in Aedes mosquitoes could undermine efforts to control arboviruses. The present study aims to assess in some communes of Southern Benin, the susceptibility level of Aedes aegypti (Linnaeus, 1762) and Aedes albopictus (Skuse, 1894) to insecticides commonly used in public health, as well as mechanisms involved.

METHODS

Females Ae. albopictus and Ae. aegypti collected in Ifangni, Porto-Novo, Avrankou, Adjarra and Kétou from June 2021 to October 2022, were exposed to: deltamethrin 0.05%, permethrin 0.75%, alpha-cypermethrin 0.05%, pirimiphos methyl 0.25% and bendiocarb 0.1%, following the standard WHO susceptibility tube test protocol. In some sites, pre-exposure to the synergist PBO was used to verify if pyrethroid resistance of populations of Aedes was mediated by oxidases.

RESULTS

Full susceptibility to deltamethrin and permethrin was observed in all tested populations of Ae. albopictus. However, with alphacypermethrin, a suspected resistance was observed in Adjarra (94.67%), Ifangni (93%) and Porto-Novo (94%), and a resistance in Avrankou (83%). The PBO-alphacypermethrin tests performed, led to a full susceptibility (100%) in all four sites, which confirms the full involvement of oxidases in resistance of all tested populations of Ae. albopictus to alphacypermethrin. At the opposite, Aedes aegypti was either resistant or suspected of being resistant to all tested pyrethroids in all four sites, except in Ifangni where a full susceptibility to alphacypermethrin was observed. The full susceptibility of Ae. aegypti to bendiocarb and pirimiphos-methyl in all communes suggests that these two insecticides can be good candidates for an effective control of pyrethroid-resistant Aedes vector populations. Use of permethrin and deltamethrin could also be considered for controlling populations of Ae. albopictus.

CONCLUSION

Results of the present study will help guide strategy to implement for an effective control of Aedes vector populations in Benin.

摘要

背景

伊蚊对杀虫剂产生抗药性可能会破坏控制虫媒病毒的努力。本研究旨在评估在贝宁南部的一些社区中，埃及伊蚊（林奈，1762年）和白纹伊蚊（斯库斯，1894年）对公共卫生中常用杀虫剂的敏感水平以及相关机制。

方法

2021年6月至2022年10月在伊方尼、波多诺伏、阿夫兰库、阿贾拉和凯图收集的白纹伊蚊和埃及伊蚊雌蚊，按照世界卫生组织标准敏感管试验方案，接触0.05%的溴氰菊酯、0.75%的氯菊酯、0.05%的高效氯氰菊酯、0.25%的甲基嘧啶磷和0.1%的残杀威。在一些地点，预先接触增效剂胡椒基丁醚（PBO）以验证伊蚊种群对拟除虫菊酯的抗性是否由氧化酶介导。

结果

在所有测试的白纹伊蚊种群中均观察到对溴氰菊酯和氯菊酯完全敏感。然而，对于高效氯氰菊酯，在阿贾拉（94.67%）、伊方尼（93%）和波多诺伏（94%）观察到疑似抗性，在阿夫兰库（83%）观察到抗性。进行的PBO - 高效氯氰菊酯试验在所有四个地点均导致完全敏感（100%），这证实氧化酶完全参与了所有测试的白纹伊蚊种群对高效氯氰菊酯的抗性。相反，埃及伊蚊在所有四个地点对所有测试的拟除虫菊酯均有抗性或疑似有抗性，除了在伊方尼观察到对高效氯氰菊酯完全敏感。在所有社区中埃及伊蚊对残杀威和甲基嘧啶磷完全敏感，这表明这两种杀虫剂可能是有效控制对拟除虫菊酯有抗性的伊蚊媒介种群的良好候选药物。也可考虑使用氯菊酯和溴氰菊酯来控制白纹伊蚊种群。