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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Frighi V, Ng L L, Lewis A, Dhar H

Sheikh Rashid Diabetes Unit, Radcliffe Infirmary, Oxford, U.K.

Clin Sci (Lond). 1991 Feb;80(2):95-9. doi: 10.1042/cs0800095.

Na+/H+ antiport activity was measured in peripheral blood polymorphonuclear and mononuclear cells of 12 healthy subjects by using an intracellular pH clamp technique to determine the external Na(+)-dependent H+ efflux rate in cells loaded with a pH-sensitive fluorescent dye, bis(carboxyethyl)carboxyfluorescein. The change in external Na+ concentrations for all pH measurements was similar in both cell types. 2. A significant difference between the two types of cells was found, the polymorphonuclear leucocytes having a higher Na+/H+ antiport activity than the lymphocytes. Cellular intrinsic buffering capacity measured in the absence of HCO3- was also higher in the polymorphonuclear cells than in the lymphocytes. 3. These differences may be associated with a difference in the role of the Na+/H+ exchanger in these two types of cells, although in vivo the presence of HCO3-/Cl- exchangers may also contribute to intracellular pH homoeostasis.

通过使用细胞内pH钳技术，在12名健康受试者的外周血多形核细胞和单核细胞中测量Na⁺/H⁺逆向转运活性，以确定加载有pH敏感荧光染料双（羧乙基）羧基荧光素的细胞中依赖外部Na⁺的H⁺外流速率。在两种细胞类型中，所有pH测量的外部Na⁺浓度变化相似。2. 发现两种细胞类型之间存在显著差异，多形核白细胞的Na⁺/H⁺逆向转运活性高于淋巴细胞。在不存在HCO₃⁻的情况下测量的细胞固有缓冲能力在多形核细胞中也高于淋巴细胞。3. 这些差异可能与这两种细胞类型中Na⁺/H⁺交换器的作用差异有关，尽管在体内HCO₃⁻/Cl⁻交换器的存在也可能有助于细胞内pH稳态。

Frighi V, Ng L L, Lewis A, Dhar H

Sheikh Rashid Diabetes Unit, Radcliffe Infirmary, Oxford, U.K.

Clin Sci (Lond). 1991 Feb;80(2):95-9. doi: 10.1042/cs0800095.

Na+/H+ antiport activity was measured in peripheral blood polymorphonuclear and mononuclear cells of 12 healthy subjects by using an intracellular pH clamp technique to determine the external Na(+)-dependent H+ efflux rate in cells loaded with a pH-sensitive fluorescent dye, bis(carboxyethyl)carboxyfluorescein. The change in external Na+ concentrations for all pH measurements was similar in both cell types. 2. A significant difference between the two types of cells was found, the polymorphonuclear leucocytes having a higher Na+/H+ antiport activity than the lymphocytes. Cellular intrinsic buffering capacity measured in the absence of HCO3- was also higher in the polymorphonuclear cells than in the lymphocytes. 3. These differences may be associated with a difference in the role of the Na+/H+ exchanger in these two types of cells, although in vivo the presence of HCO3-/Cl- exchangers may also contribute to intracellular pH homoeostasis.

通过使用细胞内pH钳技术，在12名健康受试者的外周血多形核细胞和单核细胞中测量Na⁺/H⁺逆向转运活性，以确定加载有pH敏感荧光染料双（羧乙基）羧基荧光素的细胞中依赖外部Na⁺的H⁺外流速率。在两种细胞类型中，所有pH测量的外部Na⁺浓度变化相似。2. 发现两种细胞类型之间存在显著差异，多形核白细胞的Na⁺/H⁺逆向转运活性高于淋巴细胞。在不存在HCO₃⁻的情况下测量的细胞固有缓冲能力在多形核细胞中也高于淋巴细胞。3. 这些差异可能与这两种细胞类型中Na⁺/H⁺交换器的作用差异有关，尽管在体内HCO₃⁻/Cl⁻交换器的存在也可能有助于细胞内pH稳态。