兔肾中神经肽Y受体蛋白的鉴定。G蛋白偶联及腺苷酸环化酶的抑制作用。

Identification of a receptor protein for neuropeptide Y in rabbit kidney. G-protein association and inhibition of adenylate cyclase.

作者信息

Gimpl G, Wahl J, Lang R E

机构信息

Department of Pharmacology, University of Heidelberg, Germany.

出版信息

FEBS Lett. 1991 Feb 25;279(2):219-22. doi: 10.1016/0014-5793(91)80153-t.

DOI:10.1016/0014-5793(91)80153-t

PMID:1848189

Abstract

We previously identified the receptor for neuropeptide Y (NPY) on rabbit kidney membranes as a 100 kDa protein [(1990) J. Biol. Chem. 265, 18142-18143]. It is demonstrated in this study that the stable guanine nucleotide analogue, guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S), is capable of decreasing the labeling of the 100 kDa protein in a concentration-dependent manner, with an IC50 value of 50 microM. This suggests that the 100 kDa protein represents a G-protein coupled receptor type. In isolated proximal tubules from rabbit kidney NPY decreases the parathyroid hormone stimulated cAMP production in a dose-dependent fashion. This indicates that the NPY receptor in rabbit kidney is negatively coupled to adenylate cyclase by a G1-like protein.

摘要

我们之前已鉴定出兔肾膜上神经肽Y（NPY）的受体是一种100 kDa的蛋白质[（1990年）《生物化学杂志》265卷，18142 - 18143页]。本研究表明，稳定的鸟嘌呤核苷酸类似物鸟苷5'-O-（3-硫代三磷酸）（GTPγS）能够以浓度依赖的方式降低100 kDa蛋白质的标记，IC50值为50μM。这表明100 kDa蛋白质代表一种G蛋白偶联受体类型。在兔肾分离的近端小管中，NPY以剂量依赖的方式降低甲状旁腺激素刺激的cAMP产生。这表明兔肾中的NPY受体通过一种类似G1的蛋白质与腺苷酸环化酶负偶联。