Liu Xiaoyan, Ji Yongsheng, Zhang Haixia, Liu Mancang
College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2008 Jun;25(6):772-8. doi: 10.1080/02652030701713921.
Solid-phase microextraction coupled to high-performance liquid chromatography (SPME-HPLC) with fluorescence detection was employed to determine bisphenol A (BPA) in milk samples. The potential influence of the milk matrix on the determination of BPA by SPME-HPLC were investigated. Optimal conditions to eliminate any matrix effects were as follows: milk samples were deproteinized with trichloroacetic acid, diluted 20-fold with BPA-free Ultrapure water, dissolved in methanol, the precipitated protein was filtered out, rinsed with methanol and evaporated to remove the methanol. Then, a 40.0-ml solution was used for SPME extraction and HPLC analysis. Satisfactory recoveries (milk: 93.1-101%; soybean milk: 93.9-102%) were achieved. The proposed method was successfully applied to real samples, BPA being detected within the range 1.6-2.6 ng ml(-1) in four brands of commercial milk but not in soybean milk.
采用固相微萃取结合高效液相色谱法（SPME-HPLC）和荧光检测法测定牛奶样品中的双酚A（BPA）。研究了牛奶基质对SPME-HPLC测定BPA的潜在影响。消除任何基质效应的最佳条件如下：用三氯乙酸对牛奶样品进行脱蛋白处理，用无BPA的超纯水稀释20倍，溶解于甲醇中，过滤掉沉淀的蛋白质，用甲醇冲洗并蒸发以除去甲醇。然后，取40.0 ml溶液用于SPME萃取和HPLC分析。回收率令人满意（牛奶：93.1-101%；豆浆：93.9-102%）。该方法成功应用于实际样品，在四个品牌的市售牛奶中检测到BPA的含量在1.6-2.6 ng ml(-1)范围内，而豆浆中未检测到。
