利用扩增不应性突变系统（ARMS）对白花石斛进行分子鉴定。

Molecular authentication of Dendrobium loddigesii Rolfe by Amplification Refractory Mutation System (ARMS).

作者信息

Qian Liang, Ding Ge, Zhou Qi, Feng Zhenyu, Ding Xiaoyu, Gu Sun, Wang Ying, Li Xuexia, Chu Bihai

机构信息

Jiangsu Key Laboratory for Biodiversity & Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, P. R. China.

出版信息

Planta Med. 2008 Mar;74(4):470-3. doi: 10.1055/s-2008-1034360.

DOI:10.1055/s-2008-1034360

PMID:18484545

Abstract

As a widely used medicinal plant, Dendrobium loddigesii Rolfe is always a possible target for fraudulent labeling. The identification of D. loddigesii is generally difficult from its morphological and chemical appearance only. In order to develop a convenient and efficient identification method for D. loddigesii, six pairs of diagnostic ARMS primers were designed based on nrDNA ITS sequences of D. loddigesii and eleven adulterants. The results showed that one diagnostic primer pair (FJB-04-forward, FJB-04-reverse) could be used to authenticate D. loddigesii by generating a fragment of 353 bp at annealing temperatures from 48 degrees C to 62 degrees C while the other diagnostic primer pair (FJB-03-forward, FJB-03-reverse) took on the same effect at annealing temperatures from 49 degrees C to 55 degrees C. This points out the potential of ARMS analysis for authentication of D. loddigesii.

摘要

作为一种广泛使用的药用植物，滇金石斛一直是标签欺诈的潜在目标。仅从形态和化学外观上识别滇金石斛通常很困难。为了开发一种方便、高效的滇金石斛鉴定方法，基于滇金石斛及其11种掺伪品的nrDNA ITS序列设计了6对诊断性扩增受阻突变系统（ARMS）引物。结果表明，一对诊断引物（FJB-04正向引物，FJB-04反向引物）可用于鉴定滇金石斛，在48℃至62℃的退火温度下产生353 bp的片段，而另一对诊断引物（FJB-03正向引物，FJB-03反向引物）在49℃至55℃的退火温度下具有相同效果。这表明了ARMS分析在滇金石斛鉴定中的潜力。