人类T细胞对β-半乳糖苷酶的反应。
Human T cell responses to beta-galactosidase.
作者信息
Oxley J D, Brookes R H, Rayfield L S, Shepherd P S
机构信息
Department of Immunology, United Medical School, Guy's Hospital, London, England.
出版信息
Clin Exp Immunol. 1991 Mar;83(3):505-9. doi: 10.1111/j.1365-2249.1991.tb05669.x.
The peripheral blood of most normal individuals has been shown to contain T cells that respond to beta-galactosidase (beta-Gal), presumably as a result of natural priming. Three T cell clones (clones 1,2,4) specific for beta-Gal were isolated from peripheral blood mononuclear cells (PBMC) after pretreatment with leucine methyl ester (LeuOMe); a fourth clone from the same individual was isolated from untreated cells. All four clones were CD4+ CD8- alpha beta TcR+ and clone 1 was additionally shown to be cytotoxic. Epstein-Barr virus (EBV) transformed B cell lines were derived from LeuOMe-treated or untreated PBMC and used to study the efficiency of presentation of beta-Gal to one of the clones. The results indicated that B cells transformed after LeuOMe treatment presented beta-Gal at lower concentrations than untreated controls. beta-Gal would therefore appear to be a highly suitable model antigen for studies of immunoregulation in humans.
多数正常个体的外周血已被证明含有对β-半乳糖苷酶（β-Gal）产生反应的T细胞，推测这是自然致敏的结果。在用亮氨酸甲酯（LeuOMe）预处理后，从外周血单个核细胞（PBMC）中分离出三个对β-Gal特异的T细胞克隆（克隆1、2、4）；从同一个体未处理的细胞中分离出第四个克隆。所有四个克隆均为CD4+ CD8-αβ TcR+，并且克隆1还显示具有细胞毒性。爱泼斯坦-巴尔病毒（EBV）转化的B细胞系源自经LeuOMe处理或未处理的PBMC，并用于研究将β-Gal呈递给其中一个克隆的效率。结果表明，LeuOMe处理后转化的B细胞在比未处理的对照更低的浓度下呈递β-Gal。因此，β-Gal似乎是用于人类免疫调节研究的高度合适的模型抗原。
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