Rayfield L S, Brent L, Rodeck C H
Clin Exp Immunol. 1980 Dec;42(3):561-70.
White cells from pure foetal blood obtained by fetoscopy, or from the cord at birth, were sensitized in mixed lymphocyte culture with irradiated adult peripheral blood lymphocytes. After 6-8 days of culture they were assayed in a standard 4-hr 51Cr-release cytotoxicity assay (CML) using lymphoblastoid cell targets from the stimulator cell donor. The age of the foetuses ranged from 15-22 weeks of gestation. Third-party target cells and adult blood served as controls. The mean cytotoxic responses of cord (11 donors) and adult (10 donors) blood lymphocytes were not significantly different and had similar kinetics. Foetal lymphocytes (25 donors) displayed a wide range of reactivity with half, scattered throughout the age range, totally negative and a further 23% with marginal responses. Definite cytotoxicity was found in the remainder, but not before the 18th week; these responses were evenly distributed in the range 18-22 weeks. Third-party responses were never more than one-quarter of the specific cytotoxicity. It was shown that the negative and very weak responses were almost certainly not due to technical factors. It is concluded that the majority of human foetuses in the range 15-22 weeks, though capable of giving clear mixed lymphocyte reactions, cannot develop full effector function as measured by the CML assay.
通过胎儿镜检查获取的纯胎儿血液中的白细胞，或出生时脐带血中的白细胞，在混合淋巴细胞培养中与经辐照的成人外周血淋巴细胞发生致敏反应。培养6 - 8天后，使用来自刺激细胞供体的淋巴母细胞样细胞靶标，在标准的4小时51铬释放细胞毒性试验（细胞介导的淋巴细胞毒试验）中对其进行检测。胎儿的孕周范围为15 - 22周。第三方靶细胞和成人血液用作对照。脐带血（11名供体）和成人血（10名供体）淋巴细胞的平均细胞毒性反应无显著差异，且动力学相似。胎儿淋巴细胞（25名供体）表现出广泛的反应性，其中一半在整个孕周范围内呈散在分布，完全无反应，另有23%反应微弱。其余胎儿淋巴细胞有明确的细胞毒性，但在第18周之前未发现；这些反应在18 - 22周范围内均匀分布。第三方反应从未超过特异性细胞毒性的四分之一。结果表明，阴性和非常微弱的反应几乎肯定不是技术因素所致。得出的结论是，15 - 22周范围内的大多数人类胎儿，尽管能够产生明显的混合淋巴细胞反应，但通过细胞介导的淋巴细胞毒试验检测，其无法发育出完全的效应功能。
