Yeh Shinn-Pyng, Chang Chen-An, Chang Chi-Yao, Liu Chun-Hung, Cheng Winton
Department of Aquaculture, National Pingtung University of Science and Technology, Pingtung, Taiwan.
Fish Shellfish Immunol. 2008 Jul;25(1-2):19-27. doi: 10.1016/j.fsi.2007.11.011. Epub 2007 Dec 3.
The percent weight gain (PWG) and feeding efficiency (FE) of fingerling orange-spotted grouper, Epinephelus coioides, fed diets containing sodium alginate at 1.0 and 2.0 g kg(-1) were calculated on the 2nd, 4th, 6th, and 8th weeks after feeding. Survival rates of the fingerling grouper against Streptococcus sp. and an iridovirus, and non-specific immune parameters such as alternative complement activity (ACH50), lysozyme activity, natural haemagglutination activity, respiratory bursts, superoxide dismutase (SOD) activity, and phagocytic activity of juvenile grouper were also determined when the fish were fed diets containing sodium alginate at 0.5, 1.0, or 2.0 g kg(-1). The PWG and FE of fish were better when the fish were fed diets containing sodium alginate at 1.0, and 1.0 and 2.0 g kg(-1), respectively. The PWG and FE of fish fed the 0, 1.0 and 2.0 g kg(-1) sodium alginate-containing diets after 8 weeks were 271.0%, 454.4% and 327.8%, and 0.61, 0.72 and 0.68, respectively. Fish fed a diet containing sodium alginate at the level of 2.0 g kg(-1) had a significantly higher survival rate than those fed the control diet after challenge with Streptococcus sp. and an iridovirus causing an increase of survival rate by 25.0% and 16.7%, respectively, compared to the control group. The ACH(50) level of fish fed the sodium alginate-containing diets at 2.0 g kg(-1) was significantly higher than those fed the 1.0 g kg(-1) sodium alginate diet and control diet after 12 days, and had increased to 1.9-fold, compared to those fed the control diet. The lysozyme activity, phagocytic activity, respiratory bursts, and SOD level of fish fed the sodium alginate-containing diets at 1.0 and 2.0 g kg(-1) were significantly higher than those fed the control diet after 12 days, and had increased to 1.97- and 1.68-fold, 1.35- and 1.50-fold, 1.63- and 1.81-fold, and 1.23- and 1.31-fold, respectively, compared to those fed the control diet. We therefore recommend dietary sodium alginate administration at 1.0 and 2.0 g kg(-1), respectively, to promote growth and enhance immunity and resistance against Streptococcus sp. and an iridovirus.
在投喂后第2、4、6和8周，计算投喂含1.0和2.0 g kg⁻¹海藻酸钠饲料的点带石斑鱼幼鱼的体重增加百分比（PWG）和投喂效率（FE）。当幼鱼投喂含0.5、1.0或2.0 g kg⁻¹海藻酸钠的饲料时，还测定了其对链球菌属和一种虹彩病毒的存活率，以及幼鱼的非特异性免疫参数，如替代补体活性（ACH50）、溶菌酶活性、自然血凝活性、呼吸爆发、超氧化物歧化酶（SOD）活性和吞噬活性。当鱼分别投喂含1.0 g kg⁻¹以及1.0和2.0 g kg⁻¹海藻酸钠的饲料时，其PWG和FE较好。投喂8周后，投喂含0、1.0和2.0 g kg⁻¹海藻酸钠饲料的鱼的PWG分别为271.0%、454.4%和327.8%，FE分别为0.61、0.72和0.68。在受到链球菌属和一种虹彩病毒攻击后，投喂含2.0 g kg⁻¹海藻酸钠饲料的鱼的存活率显著高于投喂对照饲料的鱼，与对照组相比，存活率分别提高了25.0%和16.7%。在12天后，投喂含2.0 g kg⁻¹海藻酸钠饲料的鱼的ACH(50)水平显著高于投喂含1.0 g kg⁻¹海藻酸钠饲料和对照饲料的鱼，与投喂对照饲料的鱼相比，增加到了1.9倍。在12天后，投喂含1.0和2.0 g kg⁻¹海藻酸钠饲料的鱼的溶菌酶活性、吞噬活性、呼吸爆发和SOD水平显著高于投喂对照饲料的鱼，与投喂对照饲料的鱼相比，分别增加到了1.97倍和1.68倍、1.35倍和1.50倍、1.63倍和1.81倍、1.23倍和1.31倍。因此，我们建议分别以1.0和2.0 g kg⁻¹的剂量投喂海藻酸钠，以促进生长并增强对链球菌属和一种虹彩病毒的免疫力和抵抗力。
