Luchsinger Vivian, Noy Andrea Elgueta, Avendaño Luis F
Programa de Virología, ICBM, Facultad de Medicina, Universidad de Chile, Independencia 1027, Santiago, Chile.
J Clin Virol. 2008 Jul;42(3):260-3. doi: 10.1016/j.jcv.2008.03.024. Epub 2008 May 15.
Human respiratory syncytial virus (HRSV) is a major cause of severe lower respiratory tract infection (LRI) in children. Distinct variants of the viruses have been described.
The objective was to compare the antigenic and genetic variability of HRSV strains recovered from infants admitted to two hospitals during one epidemic in a big city.
We analyzed nasopharyngeal aspirates from 201 infants admitted for LRI to two hospitals during 2002 in Santiago, Chile. The analyses were carried out using a panel of monoclonal antibodies against G glycoprotein epitopes (EIA) and RFLP for N and G genes.
No differences in HRSV groups A/B and in N patterns distribution were observed among both hospitals. On the contrary, antigenic and genetic G patterns displayed a wide diversity of strains circulating during one epidemic, in one big city.
RSV variability assessment depended rather on the tool used for analysis than on the geographical location.
人呼吸道合胞病毒（HRSV）是导致儿童严重下呼吸道感染（LRI）的主要原因。已描述了该病毒的不同变体。
目的是比较在一个大城市的一次流行期间从两家医院收治的婴儿中分离出的HRSV毒株的抗原性和基因变异性。
我们分析了2002年在智利圣地亚哥两家医院因LRI收治的201名婴儿的鼻咽抽吸物。使用一组针对G糖蛋白表位的单克隆抗体（酶免疫测定）以及对N和G基因进行限制性片段长度多态性分析。
两家医院在HRSV A/B组和N型分布方面未观察到差异。相反，在一个大城市的一次流行期间，抗原性和基因G型显示出循环毒株的广泛多样性。
RSV变异性评估更多地取决于所使用的分析工具，而非地理位置。
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