Zhang Y, Xu W, Shen K, Xie Z, Sun L, Lu Q, Liu C, Liang G, Beeler J A, Anderson L J
China Center for Disease Control and Prevention, National Institute for Viral Disease Control and Prevention, Beijing, China.
Arch Virol. 2007;152(8):1425-34. doi: 10.1007/s00705-007-0984-3. Epub 2007 May 18.
The genetic variability of HRSV in China was studied using nucleotide sequencing of the hypervariable C-terminal region of the G protein gene and phylogenetic analysis on 80 isolates obtained from three children's hospitals over a period of three epidemic seasons, 1990/1991, 2000/2001, and 2003/2004. The results showed that 76/80 of these isolates belonged to group A and 4/80 belonged to group B. Phylogenetic analysis revealed that most of the group A isolates were genotype GA2 (74/76 isolates), and the other two isolates were GA3 and GA5. All group B isolates clustered into genotype GB3. There was substantial variation among the GA2 isolates, with nucleotide sequence and amino acid homologies ranging from 88.1-100% and 78.4-100%, respectively, in the hypervariable C-terminal region of the G protein gene. One group B virus, HRSV/Beijing/B/04/11, contained a 60-nucleotide duplication in the C-terminal region of the G protein, which was similar to what has been reported previously for isolates in several countries. This is the first report on the genetic diversity of human respiratory syncytial virus isolated during epidemic periods from children in China. These data provided a preliminary evaluation of patterns of circulation and the genetic diversity of isolates associated with HRSV epidemics within China.
利用G蛋白基因高变C末端区域的核苷酸测序技术以及系统发育分析，对1990/1991、2000/2001和2003/2004三个流行季节从三家儿童医院采集的80株呼吸道合胞病毒（HRSV）分离株进行研究，以了解中国HRSV的基因变异性。结果显示，这些分离株中76/80属于A组，4/80属于B组。系统发育分析表明，大多数A组分离株为GA2基因型（74/76株），另外两株为GA3和GA5。所有B组分离株均聚类为GB3基因型。GA2分离株之间存在显著差异，在G蛋白基因高变C末端区域，核苷酸序列和氨基酸同源性分别为88.1%-100%和78.4%-100%。一株B组病毒HRSV/Beijing/B/04/11在G蛋白C末端区域存在60个核苷酸的重复，这与此前在其他几个国家报道的分离株情况相似。这是关于中国儿童流行期分离的人呼吸道合胞病毒基因多样性的首次报道。这些数据对中国境内与HRSV流行相关的病毒株的传播模式和基因多样性进行了初步评估。
