Frabasile Sandra, Delfraro Adriana, Facal Luján, Videla Cristina, Galiano Mónica, de Sierra María José, Ruchansky Dora, Vitureira Nathalia, Berois Mabel, Carballal Guadalupe, Russi José, Arbiza Juan
Sección Virología, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay.
J Med Virol. 2003 Oct;71(2):305-12. doi: 10.1002/jmv.10484.
The antigenic and genetic diversity of G glycoprotein from 25 human respiratory viruses (group A) isolated during nine consecutive epidemics (1993-2001) in Montevideo, Uruguay, and 7 strains isolated in Buenos Aires, Argentina, in the same period were analyzed. Genetic variability was evaluated by partial sequence of the G protein gene. Phylogenetic analysis indicated that most Uruguayan and Argentinean group A isolates clustered into three genotypes: GA5, GA2, and GA1. Some strains clustered into the GA3 genotype characterized previously. The antigenic analysis was carried out with a panel of anti-G monoclonal antibodies that recognized conserved and strain-specific epitopes. A close correlation between the antigenic and genetic relatedness of the strains analyzed was observed.
对1993年至2001年在乌拉圭蒙得维的亚连续9次流行期间分离出的25株人类呼吸道病毒（A组）以及同期在阿根廷布宜诺斯艾利斯分离出的7株病毒的G糖蛋白的抗原性和基因多样性进行了分析。通过G蛋白基因的部分序列评估基因变异性。系统发育分析表明，大多数乌拉圭和阿根廷A组分离株聚为三个基因型：GA5、GA2和GA1。一些菌株聚为先前鉴定的GA3基因型。使用一组识别保守和菌株特异性表位的抗G单克隆抗体进行抗原分析。观察到所分析菌株的抗原性和基因相关性之间存在密切关联。
