Poidinger M, Coelen R J, Mackenzie J S
Department of Microbiology, Queen Elizabeth II Medical Centre, University of Western Australia, Nedlands.
J Gen Virol. 1991 Mar;72 ( Pt 3):573-8. doi: 10.1099/0022-1317-72-3-573.
Murray Valley encephalitis (MVE) virus strain OR2 was serially passaged on Vero cells to establish a persistent infection which was maintained for over 300 days. Supernatants from infected cells protected Vero cells from c.p.e. and caused up to a 95% reduction of wild-type virus yield. These protective and interfering effects suggest that defective interfering (DI) particles are responsible for the establishment and maintenance of the MVE virus persistent infection. The persistently infected cell supernatant preparations shared several features with DI particle preparations from other viral systems, such as their amplification to detectable levels after two to four passages of virus. However, results from this study suggest that DI particles of MVE virus differ from other studied systems in that they are able to affect only moderately the yield of infectious wild-type virus. The genetic drift of the parental virus during the course of a long term persistent infection in vitro appears to be minimal.
墨累谷脑炎（MVE）病毒OR2株在Vero细胞上连续传代以建立持续感染，该感染维持了300多天。感染细胞的上清液保护Vero细胞免受细胞病变效应影响，并使野生型病毒产量降低达95%。这些保护和干扰作用表明，缺陷干扰（DI）颗粒是MVE病毒持续感染建立和维持的原因。持续感染细胞的上清液制剂与来自其他病毒系统的DI颗粒制剂有几个共同特征，例如在病毒传代两到四次后它们扩增到可检测水平。然而，本研究结果表明，MVE病毒的DI颗粒与其他研究系统不同，因为它们仅能适度影响感染性野生型病毒的产量。在体外长期持续感染过程中，亲代病毒的基因漂移似乎极小。
