Barr J, Chambers P, Pringle C R, Easton A J
Department of Biological Sciences, University of Warwick, Coventry, U.K.
J Gen Virol. 1991 Mar;72 ( Pt 3):677-85. doi: 10.1099/0022-1317-72-3-677.
The complete nucleotide sequence of gene 3 of pneumonia virus of mice has been determined, and the 5' end of the mRNA mapped using a modification of the polymerase chain reaction technique. The gene contains a single open reading frame, beginning with a 5'-proximal AUG initiation codon, encoding a polypeptide with a predicted Mr of 43141. Expression of the gene 3 protein in Escherichia coli and in vitro showed that it reacted with virus-specific antiserum and comigrated with the major nucleocapsid (N) polypeptide. The predicted amino acid sequence has extensive identity with that of the N protein of human respiratory syncytial virus. Comparisons with the amino acid sequences of N proteins of other paramyxoviruses, vesicular stomatitis virus and Ebola virus suggest that these proteins may have retained much of the same structure. These regions of conserved structure would most likely have the common functions of RNA binding and protein/protein interactions in the virus nucleocapsid.
已确定小鼠肺炎病毒基因3的完整核苷酸序列，并使用聚合酶链反应技术的改进方法绘制了mRNA的5'末端图谱。该基因包含一个单一的开放阅读框，起始于5'-近端AUG起始密码子，编码一个预测分子量为43141的多肽。基因3蛋白在大肠杆菌中的表达以及体外实验表明，它与病毒特异性抗血清发生反应，并与主要核衣壳（N）多肽共迁移。预测的氨基酸序列与人类呼吸道合胞病毒的N蛋白有广泛的同源性。与其他副粘病毒、水泡性口炎病毒和埃博拉病毒的N蛋白的氨基酸序列比较表明，这些蛋白可能保留了许多相同的结构。这些保守结构区域很可能在病毒核衣壳中具有RNA结合和蛋白质/蛋白质相互作用的共同功能。
