Spriggs M K, Collins P L
J Gen Virol. 1986 Dec;67 ( Pt 12):2705-19. doi: 10.1099/0022-1317-67-12-2705.
The complete nucleotide sequence of the P + C mRNA of human parainfluenza virus type 3 (PF3) was determined by sequencing cDNA, viral genomic RNA and mRNA. The P + C mRNA is 2009 nucleotides in length, exclusive of poly(A), and contains two overlapping open reading frames (ORFs). The P + C mRNA encodes two proteins, the 602 amino acid nucleocapsid phosphoprotein P and the 199 amino acid non-structural protein C. Peptide mapping confirmed that the two proteins are unrelated. Hybrid-arrest translation experiments assigned each of the two proteins to its respective ORF. These studies showed that the coding strategy of the PF3 P + C mRNA is similar to that of Sendai virus. Amino acid sequence alignment showed that the P and C proteins of PF3 and Sendai virus represent homologous pairs. However, these homologies are represented by high contents of accepted amino acid substitutions and by similarity in hydropathy profiles rather than by high contents of exact amino acid matches. Homology with the P and C proteins of measles, canine distemper and respiratory syncytial viruses was at the threshold of significance. The patterns of amino acid sequence homology among the paramyxovirus HN, F, NP, P and C proteins are compared.
通过对互补DNA（cDNA）、病毒基因组RNA和信使核糖核酸（mRNA）进行测序，确定了人副流感病毒3型（PF3）的P + C mRNA的完整核苷酸序列。P + C mRNA长度为2009个核苷酸（不包括聚腺苷酸），包含两个重叠的开放阅读框（ORF）。P + C mRNA编码两种蛋白质，即602个氨基酸的核衣壳磷蛋白P和199个氨基酸的非结构蛋白C。肽图谱分析证实这两种蛋白质没有关联。杂交阻断翻译实验将这两种蛋白质分别定位到各自的开放阅读框。这些研究表明，PF3的P + C mRNA的编码策略与仙台病毒相似。氨基酸序列比对显示，PF3和仙台病毒的P蛋白和C蛋白是同源对。然而，这些同源性表现为可接受的氨基酸取代含量高以及亲水性图谱相似，而不是精确氨基酸匹配的含量高。与麻疹病毒、犬瘟热病毒和呼吸道合胞病毒的P蛋白和C蛋白的同源性处于显著水平的临界值。比较了副粘病毒血凝素神经氨酸酶（HN）、融合蛋白（F）、核蛋白（NP）、磷蛋白（P）和非结构蛋白（C）之间的氨基酸序列同源性模式。
