Faculty of Agriculture, University of South Bohemia in České Budějovice, Studentská 13, 370 05 České Budějovice, Czech Republic.
Vet Parasitol. 2013 Nov 8;197(3-4):504-8. doi: 10.1016/j.vetpar.2013.07.003. Epub 2013 Jul 12.
From 2011 to 2012, to identify Cryptosporidium spp. occurrence in Eurasian wild boars (Sus scrofa) 29 randomly selected localities (both forest areas and enclosures) across the Central European countries of Austria, the Czech Republic, Poland, and the Slovak Republic were investigated. Cryptosporidium oocysts were microscopicaly detected in 11 out of 460 faecal samples examined using aniline-carbol-methyl violet staining. Sixty-one Cryptosporidium infections, including the 11 infections that were detected by microscopy, were detected using genus- or species-specific nested PCR amplification of SSU rDNA. This represents a 5.5 fold greater sensitivity for PCR relative to microscopy. Combining genus- and species-specific PCR tools significantly changes the perspective on the occurrence of Cryptosporidium spp. in wild boars. While RFLP and direct sequencing of genus specific PCR-amplified products revealed 56 C. suis (20) and C. scrofarum (36) monoinfections and only 5 mixed infections of these species, species-specific molecular tools showed 44 monoinfections and 17 mixed infections with these species. PCR analysis of the gp60 gene did not reveal any other Cryptosporidium infections. Similar to domestic pigs, C. scrofarum was detected as a dominant species infecting adult Eurasian wild boars (Sus scrofa). Cryptosporidium infected wild boars did not show signs of clinical disease. This report is perhaps the most comprehensive survey of cryptosporidial infection in wild boars.
从 2011 年到 2012 年，为了确定中 欧 4 国（奥地利、捷克共和国、波兰和斯洛伐克共和国）的欧洲野猪（Sus scrofa）中隐孢子虫属的发生情况，对 29 个随机选择的地点（森林地区和围栏）进行了调查。用苯胺-咔唑-甲基紫染色法对 460 个粪便样本进行显微镜检查，发现 11 个样本中存在隐孢子虫卵囊。用种特异性巢式 PCR 扩增 SSU rDNA 检测到 61 种隐孢子虫感染，包括 11 种通过显微镜检测到的感染。这比显微镜检测的灵敏度高 5.5 倍。种属和种特异性 PCR 工具的结合极大地改变了对野猪中隐孢子虫属发生情况的看法。虽然 RFLP 和直接测序对种特异性 PCR 扩增产物的分析显示了 56 种 C. suis（20 种）和 C. scrofarum（36 种）的单一感染，只有 5 种这些种的混合感染，但种特异性分子工具显示了 44 种单一感染和 17 种这些种的混合感染。对 gp60 基因的 PCR 分析没有显示任何其他隐孢子虫感染。与家猪相似，C. scrofarum 被检测为感染成年欧洲野猪（Sus scrofa）的主要种。感染隐孢子虫的野猪没有表现出临床疾病的迹象。本报告可能是对野猪隐孢子虫感染最全面的调查。
