sigH基因序列可对葡萄球菌进行亚种分类。
The sigH gene sequence can subspeciate staphylococci.
作者信息
Morikawa Kazuya, Ohniwa Ryosuke L, Kumano Miyuki, Okamura Hideyuki, Saito Shinji, Ohta Toshiko
机构信息
Institute of Basic Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba 305-8575, Japan.
出版信息
Diagn Microbiol Infect Dis. 2008 Aug;61(4):373-80. doi: 10.1016/j.diagmicrobio.2008.03.015. Epub 2008 May 16.
In an evolutionarily conserved gene organization (syntenic region), the sigH gene shares exceptionally low homology among staphylococcal species. We analyzed the "positionally cloned" sigH sequences of 39 staphylococcal species. The topology of the SigH phylogenetic tree was consistent with that of 16S rRNA. Certain clinical isolates were successfully differentiated at the species level with the sigH sequence data set. We propose that the sigH gene is a promising molecular target in genotypic identification because it is highly discriminative in differentiating closely related staphylococcal species.
在一个进化上保守的基因组织（同线区域）中，sigH基因在葡萄球菌物种间的同源性极低。我们分析了39种葡萄球菌物种的“定位克隆”sigH序列。SigH系统发育树的拓扑结构与16S rRNA的拓扑结构一致。利用sigH序列数据集，某些临床分离株在物种水平上成功得到区分。我们提出，sigH基因是基因型鉴定中一个有前景的分子靶点，因为它在区分密切相关的葡萄球菌物种方面具有高度鉴别力。
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