Lee Tae-Hoon, Yoon Jung-Gyu
Department of Health Administration, Namseoul University, 21 Maeju-ri, Seonghwan-eup, Cheonan-city, Choongnam 330-707, Korea.
J Clin Neurosci. 2008 Aug;15(8):907-12. doi: 10.1016/j.jocn.2007.03.016. Epub 2008 May 16.
The transplantation of cells capable of neuronal differentiation has great potential for the treatment of neurological conditions. I examined whether human adipose tissue stromal cells (hATSCs) can be induced to undergo neuronal differentiation. I isolated hATSCs from human liposuction tissue and induced neuronal differentiation using azacytidine. After neuronal induction, the hATSCs adopted a more neuronal morphology. These hATSCs were injected into the lateral ventricle of the rat brain, after which they migrated to various parts of the brain. After ischemic brain injury induced by middle cerebral artery occlusion (MCAO), a large number of cells migrated to the injured cortex. Intracerebral grafting of hATSCs significantly enhanced the recovery of functional motor deficits in MCAO rats. These data indicate that transplanted hATSCs survive, migrate and differentiate in the ischemic microenvironment and improve neurological recovery after stroke in rats.
能够进行神经元分化的细胞移植在治疗神经疾病方面具有巨大潜力。我研究了人类脂肪组织基质细胞（hATSCs）是否能被诱导进行神经元分化。我从人类抽脂组织中分离出hATSCs，并使用氮杂胞苷诱导其神经元分化。神经元诱导后，hATSCs呈现出更具神经元特征的形态。将这些hATSCs注射到大鼠脑的侧脑室后，它们迁移到大脑的各个部位。在大脑中动脉闭塞（MCAO）诱导的缺血性脑损伤后，大量细胞迁移到受损皮质。hATSCs的脑内移植显著增强了MCAO大鼠功能性运动缺陷的恢复。这些数据表明，移植的hATSCs在缺血微环境中存活、迁移并分化，改善了大鼠中风后的神经恢复。
