Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; Department of Neurology, University Hospital of Hubei Institute for Nationalities, Enshi, China.
CNS Neurosci Ther. 2012 Oct;18(10):847-54. doi: 10.1111/j.1755-5949.2012.00382.x. Epub 2012 Aug 31.
To examine whether transplantation of adipose-derived stem cells (ADSCs) induces neural differentiation and improves neural function in a rat intracerebral hemorrhage (ICH) model.
Adipose-derived stem cells cells were isolated from inguinal fat pad of rat. ICH was induced by injection of collagenase type IV into the right basal ganglia of rat. Forty-eight hours after ICH, ADSCs cells (10 μL of 2-4 × 10(7) cells/mL) were injected into the right lateral cerebral ventricle. The differentiation of ADSCs was detected in vitro and in vivo. The neural function was evaluated with Zea Longa 5-grade scale at day 1, 3, 7, 14, or 28.
Our data demonstrated that ADSCs differentiated into cells that shared the similarities of neurons or astrocytes in vitro. Transplantation of ADSCs decreased cell apoptosis and the transplanted ADSCs were able to differentiate into neuron-like and astrocyte-like cells around the hematoma, accompanied with upregulation of vascular endothelial growth factor expression and improvement of neural function.
Our data suggest that transplantation of ADSCs could be a therapeutic approach for ICH stroke.
探讨脂肪来源干细胞（ADSCs）移植是否能诱导神经分化并改善脑出血（ICH）大鼠的神经功能。
从大鼠腹股沟脂肪垫中分离出脂肪来源干细胞。通过向大鼠基底节右侧注射 IV 型胶原酶诱导 ICH。ICH 后 48 小时，将 ADSCs 细胞（10 μL，浓度为 2-4×10(7)个细胞/mL）注入右侧侧脑室。在体外和体内检测 ADSCs 的分化情况。采用 Zea Longa 5 级评分标准在第 1、3、7、14 或 28 天评估神经功能。
我们的数据表明，ADSCs 可在体外分化为具有神经元或星形胶质细胞特征的细胞。移植 ADSCs 可减少细胞凋亡，并且移植的 ADSCs 能够在血肿周围分化为神经元样和星形胶质细胞样细胞，同时伴随着血管内皮生长因子表达的上调和神经功能的改善。
我们的数据表明，ADSCs 移植可能是治疗 ICH 中风的一种方法。
