Aukaty M F, Bubenschikova S N, Kagrammanova V K, Baratova L A
J Chromatogr. 1977 Jul 21;137(2):351-6. doi: 10.1016/s0021-9673(00)81358-8.
The use of an amino-silica column in the chromatographic analysis of synthetic oligodeoxyribonucleotides and their derivatives from different stages of oligonucleotide synthesis has been investigated. By eluting with 0.10 M potassium phosphate solution of pH 3.30, the nucleotide composition of oligonucleotides can be established within 15 min. In a linear gradient of phosphate buffer (0.10-0.75 M) at neutral pH, the separation of oligonucleotides by length and in an acidic medium pH 3.30-4.30) by composition is possible; the oligonucleotides may be in the free form or modified by the various protecting groups used in synthetic oligonucleotide chemistry. The analysis of some reaction mixtures from different stages of oligonucleotide synthesis and of a number of synthetic oligodeoxyribonucleotides and their derivatives has been performed.
研究了在寡核苷酸合成不同阶段的合成寡脱氧核糖核苷酸及其衍生物的色谱分析中使用氨基硅胶柱的情况。通过用pH 3.30的0.10 M磷酸钾溶液洗脱，可在15分钟内确定寡核苷酸的核苷酸组成。在中性pH下的磷酸盐缓冲液（0.10 - 0.75 M）线性梯度中，可按长度分离寡核苷酸，在pH 3.30 - 4.30的酸性介质中可按组成分离寡核苷酸；寡核苷酸可以是游离形式，也可以被合成寡核苷酸化学中使用的各种保护基团修饰。已对寡核苷酸合成不同阶段的一些反应混合物以及多种合成寡脱氧核糖核苷酸及其衍生物进行了分析。
