使用甲酰胺的寡脱氧核糖核苷酸的离子交换高效液相色谱法

Ion-exchange high-performance liquid chromatography of oligodeoxyribonucleotides using formamide.

作者信息

Newton C R, Greene A R, Heathcliffe G R, Atkinson T C, Holland D, Markham A F, Edge M D

出版信息

Anal Biochem. 1983 Feb 15;129(1):22-30. doi: 10.1016/0003-2697(83)90046-5.

Abstract

The superiority of buffer systems containing formamide for the ion-exchange high-performance liquid chromatographic separation of oligodeoxyribonucleotide mixtures generated in solid-phase syntheses is illustrated. The resolutions achieved are compared to those achieved with the same mixtures in other eluting solvents. The use of formamide systems is recommended for oligodeoxyribonucleotide purification in general and is particularly valuable where the oligonucleotide of interest is highly self-complementary and/or rich in deoxyguanosine residues.

摘要

本文阐述了含甲酰胺的缓冲体系在离子交换高效液相色谱法分离固相合成中产生的寡脱氧核糖核苷酸混合物方面的优越性。将所获得的分离度与在其他洗脱溶剂中对相同混合物进行分离所获得的分离度进行了比较。总体而言，推荐使用甲酰胺体系进行寡脱氧核糖核苷酸的纯化，对于目标寡核苷酸具有高度自互补性和/或富含脱氧鸟苷残基的情况，该体系尤为有用。