Zhou Yu, Song Bin, Qin Wei-Jun, Zhang Geng, Zhang Rui, Luan Qi, Pan Tie-Jun, Yang An-Gang, Wang He
Department of Urology, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
Cancer Lett. 2008 Sep 18;268(2):252-9. doi: 10.1016/j.canlet.2008.04.008. Epub 2008 May 19.
Heparanase is an endoglycosidase that plays an important role in angiogenesis and metastasis of cancer. Herein we evaluate the effect of heparanase overexpression on invasiveness and bone destruction in prostate cancer bone metastases. The human prostate cancer cell line PC-3 was stably transfected with a plasmid containing the cDNA for human heparanase or with the vector alone as a control. Overexpression of heparanase did not affect the growth of PC-3 cells, but did promote invasiveness of the cells in an in vitro assay. Both cell types were injected into the tibias of nude mice. Four weeks later, the mice were examined radiologically prior to sacrifice and samples of leg tissue were taken to investigate bone destruction and metastasis. Mice injected with PC-3 cells overexpressing heparanase had more severe bone destruction and larger, more invasive, tumors. These results demonstrate that heparanase overexpression can facilitate tumor invasion and accelerate bone destruction caused by prostate cancer bone metastasis.
乙酰肝素酶是一种内切糖苷酶，在癌症的血管生成和转移中起重要作用。在此，我们评估乙酰肝素酶过表达对前列腺癌骨转移中侵袭性和骨质破坏的影响。用人乙酰肝素酶cDNA质粒或单独载体作为对照稳定转染人前列腺癌细胞系PC-3。乙酰肝素酶的过表达不影响PC-3细胞的生长，但在体外试验中确实促进了细胞的侵袭性。将两种细胞类型都注射到裸鼠的胫骨中。四周后，在处死小鼠前进行放射学检查，并采集腿部组织样本以研究骨质破坏和转移情况。注射过表达乙酰肝素酶的PC-3细胞的小鼠有更严重的骨质破坏和更大、更具侵袭性的肿瘤。这些结果表明，乙酰肝素酶过表达可促进肿瘤侵袭并加速前列腺癌骨转移引起的骨质破坏。
