Yao Jun-Feng, Zhang Ying, Wu Gui-Qin, Zheng Jiang-Xia, Deng Xue-Mei, Yang Ning
College of Animal Science and Technology, China Agricultural University, Beijing 100094, China.
Yi Chuan. 2008 May;30(5):607-12. doi: 10.3724/sp.j.1005.2008.00607.
Seven single nucleotide polymorphisms (SNPs) were identified by PCR-SSCP and sequencing in the chicken apoA5 gene in F2 chickens from an experimental cross of White Plymouth Rock x Silkies. One SNP(C-169T) located on the 5'-regulatory region, another two in the second exon were transitions of C to T (600) and T to C (635). Four SNPs in the third exon were found, which were C841G, C914T, C1142G, C1394T. The association of the polymorphisms with carcass traits was investigated. The most significant results were yielded from primer apoA3F/R: the abdominal fat weight of CC chickens were significantly higher than that of AA, AB, AC, BB and BC chickens (P<0.05); AC chickens had lower liver weight than that of AA, AB, BB, BC and CC (P<0.05); BC chickens had lower heart weight than that of BB (P<0.05).
通过PCR-SSCP和测序，在白普利茅斯洛克鸡与丝羽乌骨鸡杂交F2代鸡群的apoA5基因中鉴定出7个单核苷酸多态性（SNP）。其中一个位于5'-调控区的SNP（C-169T），另外两个位于第二外显子，分别是C到T（600）和T到C（635）的转换。在第三外显子中发现了4个SNP，分别是C841G、C914T、C1142G、C1394T。研究了这些多态性与胴体性状的关联。从引物apoA3F/R获得了最显著的结果：CC基因型鸡的腹脂重量显著高于AA、AB、AC、BB和BC基因型鸡（P<0.05）；AC基因型鸡的肝脏重量低于AA、AB、BB、BC和CC基因型鸡（P<0.05）；BC基因型鸡的心脏重量低于BB基因型鸡（P<0.05）。
