College of Animal Science and Technology, Sichuan Agricultural University, Xinkang Road 46#, Ya'an, 625014, Sichuan, People's Republic of China.
Mol Biol Rep. 2010 Dec;37(8):3683-90. doi: 10.1007/s11033-010-0020-7. Epub 2010 Mar 5.
In this study, we aimed to detect the single nucleotide polymorphisms (SNPs) of the chicken FATP1 gene and discern the potential association between FATP1 SNPs and chicken carcass traits. A total of 620 meat-type quality chickens from six commercial pure lines (S01, S02, S03, S05, S06 and D99) and two cross lines (S05 × S01 and S06 × S01) were screened by using the single-strand conformational polymorphism analysis (SSCP) and DNA sequencing. Five SNPs [g.49360G > A, g.48195G > A, g.46847A > G, g.46818A > G, and g.46555A > G] were identified in chicken FATP1 gene. SNP g.46818 A > G was a rare variant and was not considered in the subsequent analysis. Sixteen haplotypes were reconstructed on the basis of the other four SNPs. The linear regression model analysis indicated that there were significant associations of certain diplotypes with part of carcass traits, such as live weight (LW), carcass weight (CW), and semi-eviscerated weight (SEW) (P < 0.05). In particular, diplotype H2H4 had a negative effect on LW, CW, SEW, and abdominal fat weight (AW); diplotype H6H10 had the highest reducing effect on subcutaneous fat thickness (SFT). Our results suggested that FATP1 gene polymorphisms were associated with chicken carcass traits or was linked with the major gene. The SNPs in this gene may be utilized as potential markers for marker-assisted selection (MAS) during chicken breeding.
在这项研究中，我们旨在检测鸡 FATP1 基因的单核苷酸多态性（SNP），并探讨 FATP1 SNP 与鸡胴体性状之间的潜在关联。我们使用单链构象多态性分析（SSCP）和 DNA 测序对来自六个商业纯系（S01、S02、S03、S05、S06 和 D99）和两个杂交系（S05×S01 和 S06×S01）的 620 只肉用型优质鸡进行了筛选。在鸡 FATP1 基因中发现了五个 SNP [g.49360G > A、g.48195G > A、g.46847A > G、g.46818A > G 和 g.46555A > G]。SNP g.46818 A > G 是一种罕见的变异，未在后续分析中考虑。基于其他四个 SNP 构建了 16 种单倍型。线性回归模型分析表明，某些二倍型与部分胴体性状存在显著关联，例如活重（LW）、胴体重量（CW）和半去内脏重（SEW）（P < 0.05）。特别是二倍型 H2H4 对 LW、CW、SEW 和腹部脂肪重量（AW）有负面影响；二倍型 H6H10 对皮下脂肪厚度（SFT）的降低效果最大。我们的结果表明，FATP1 基因多态性与鸡胴体性状相关，或与主效基因连锁。该基因中的 SNP 可作为鸡育种中辅助选择（MAS）的潜在标记。
