Zhang Chang-Qing, Wang Jin, Gao Xiang
State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China.
Yi Chuan. 2008 May;30(5):620-6. doi: 10.3724/sp.j.1005.2008.00620.
Arabidopsis TCH4 gene plays an important role in the biological processes related to plant secondary growth, resistance to pathogen, and adaptation to environmental stresses. It is up-regulated by various hormonal, environmental, and mechanical stimuli. Here, we identified 9 transcriptional regulatory elements from TCH4 promoter by bioinformatics approach. In which, 4 elements have been reported previously, and 5 elements are newly identified in this study. All of the identified elements contain the sequences of known cis-elements. Especially, their distribution along some co-expressed gene promoters and the orthologous promoters is typically clustered and syntenic. Based on our predictions and the information of known cis-elements, a model representing the transcriptional regulation mechanism was proposed for TCH4 gene in response to hormonal, mechanical, and environmental stimuli.
拟南芥TCH4基因在与植物次生生长、病原体抗性和环境胁迫适应相关的生物学过程中发挥着重要作用。它受各种激素、环境和机械刺激而上调。在此，我们通过生物信息学方法从TCH4启动子中鉴定出9个转录调控元件。其中，4个元件先前已有报道，5个元件是本研究中新鉴定的。所有鉴定出的元件都包含已知顺式元件的序列。特别是，它们沿一些共表达基因启动子和直系同源启动子的分布通常是成簇且共线的。基于我们的预测和已知顺式元件的信息，提出了一个代表TCH4基因响应激素、机械和环境刺激的转录调控机制的模型。
