Sghaier Nesrine, Ben Ayed Rayda, Gorai Mustapha, Rebai Ahmed
Laboratory of Molecular and Cellular Screening Processes, Center of Biotechnology of Sfax, B.P 1177, 3018, Sfax, Tunisia.
Faculty of Sciences of Gabes, University of Gabes, City Riadh Zerig, 6072, Gabes, Tunisia.
Mol Biol Rep. 2018 Oct;45(5):763-772. doi: 10.1007/s11033-018-4216-6. Epub 2018 Jun 23.
The plant hormone "auxin" is a key regulator of plant development and environmental responses. Many genes in Arabidopsis thaliana are known to be up-regulated in response to auxin. Auxin response factors activate or repress the expression of genes by binding at their promoter regions within auxin response elements (AuxRE) that are key regulatory cis-acting motives. Therefore, the identification of auxin-response elements is among the most important issues to understand the auxin regulation mechanisms. Thus, searching the TGTCTC motif is an unreliable method to identify AuxRE because many AuxRE variants may also be functional. In the present study, we perform an In-silico prediction of AuxREs in promoters of primary auxin responsive genes. We exploit microarray data of auxin response in Arabidopsis thaliana seedlings, in order to provide biological annotation to AuxRE. We apply a data fusion method based on the combined use of evidence theory and fuzzy sets to scan upstream sequences of response genes.
植物激素“生长素”是植物发育和环境响应的关键调节因子。已知拟南芥中的许多基因会因生长素而上调。生长素响应因子通过结合生长素响应元件（AuxRE）内的启动子区域来激活或抑制基因表达，AuxRE是关键的调控顺式作用基序。因此，生长素响应元件的鉴定是理解生长素调控机制的最重要问题之一。因此，搜索TGTCTC基序是鉴定AuxRE的不可靠方法，因为许多AuxRE变体可能也具有功能。在本研究中，我们对主要生长素响应基因启动子中的AuxRE进行了计算机预测。我们利用拟南芥幼苗生长素响应的微阵列数据，以便为AuxRE提供生物学注释。我们应用一种基于证据理论和模糊集结合使用的数据融合方法来扫描响应基因的上游序列。
