Lezhava T, Khmaladze E, Lokhadze T, Buadze T
Georgian Med News. 2008 Apr(157):72-6.
At present it is generally believed that aging is accompanied by an increase in the numbers of cells with altered chromosome sets. However, the problem of aneuploidy in extreme old age (80 years and over) has hardly been subjected to systematic investigation. The purpose of this study was to examine quantitative chromosome changes and relationship between "artefactual" and "natural" aneuploidy in subjects from 80 to 114 years of age using karyotype analyses. Chromosomes were studied in 1136 karyotypes of relatively round metaphases from 40 lymphocyte cultures obtained from 40 apparently normal subjects aged from 80 to 114 ( including 26 men and 14 women). 964 karyotypes from 48 donors aged 20 to 48 served as control. Lymphocytes were cultivation by modification method (Lezhava, 2006). It is suggested that the probability of natural aneuploidy rises substantially among elderly women; while that of artefactual aneuploidy is increased among elderly men; the problem of natural aneuploidy in males remains unclear.
目前，人们普遍认为衰老伴随着染色体组改变的细胞数量增加。然而，极端老年（80岁及以上）的非整倍体问题几乎没有得到系统的研究。本研究的目的是通过核型分析，研究80至114岁受试者的染色体数量变化以及“人为”和“自然”非整倍体之间的关系。对40名年龄在80至114岁（包括26名男性和14名女性）的明显正常受试者的40份淋巴细胞培养物中相对圆形中期的1136个核型进行了染色体研究。来自48名年龄在20至48岁的供体的964个核型作为对照。淋巴细胞采用改良方法（Lezhava，2006年）培养。研究表明，老年女性自然非整倍体的概率大幅上升；而老年男性人为非整倍体的概率增加；男性自然非整倍体问题仍不清楚。
