A histomorphometric and cytogenetic study of testis from men 29-102 years old.

作者信息

Dakouane Mbarka, Bicchieray Lucia, Bergere Marianne, Albert Martine, Vialard François, Selva Jacqueline

机构信息

Laboratoire d'Histologie Embryologie, UFR des Saint Pères, Université Paris V et UFR Paris Ile de France Ouest, Université de Versailles-Saint Quentin, Paris.

出版信息

Fertil Steril. 2005 Apr;83(4):923-8. doi: 10.1016/j.fertnstert.2004.12.005.

DOI:10.1016/j.fertnstert.2004.12.005

PMID:15820802

Abstract

OBJECTIVE

To evaluate the influence of aging on testicular histology and the aneuploidy rate in testis postmeiotic cells.

DESIGN

Comparison between older men and younger men.

SETTING

Deceased donors and patients who requested assisted reproductive technology (ART).

PATIENT(S): Thirty-six older men (61-102 years old) and 10 younger men (29-40 years old).

INTERVENTION(S): Testes were obtained from 35 deceased donors, and testicular biopsies were obtained from 11 patients who requested ART.

MAIN OUTCOME MEASURE(S): Histomorphometry of testis and fluorescent in situ hybridization (FISH), with a three-probe set X, Y, and 18.

RESULT(S): The histomorphometric study showed a thickening of the basal membrane when spermatogenesis was arrested. The number of germinal and Sertoli cells decreased as age increased. The rate of aneuploidy of postmeiotic cells was 1.1% for the control group, 1.29% for older subjects with preserved spermatogenesis, and 14.28% for the subjects with an arrested spermiogenesis. Only this last figure was higher than the control group.

CONCLUSION(S): The rate of aneuploidy in older subjects (61-95 years old) with preserved spermatogenesis was not statistically different from that found in the control group; it was increased in older subjects with arrested spermatogenesis.

摘要