Zhang HongYu, Peng Hai, Li PingChuan, Deng QiMing, Xu PeiZhou, Li Yun, Wang XuDong, Wu XianJun
Rice Research Institute of Sichuan Agricultural University, Chengdu 611130, China.
Sci China C Life Sci. 2008 Jun;51(6):503-12. doi: 10.1007/s11427-008-0061-5. Epub 2008 May 17.
In this study, microarray technique was employed to analyze the gene expression at the RNA level between haploids and corresponding diploids derived from a rice twin-seedling line SARII-628. Different degrees of expression variations were observed in the plant after haploidization. The main results are as follows: (1) after haploidization, the ratio of the sensitive loci was 2.47% of the total loci designed on chip. Those loci were randomly distributed on the 12 pairs of rice chromosomes and the activated loci were more than the silenced ones. (2) Gene clusters on chromosome were observed for 33 sequences. (3) GoPipe function classification for 575 sensitive loci revealed an involvement in the biological process, cell component and molecular function. (4) RT-PCR generally validated the result from microarray with a coincidence rate of 83.78%. And for the randomly-selected activated or silenced loci in chip analysis, the coincidence rate was up to 91.86%.
本研究采用微阵列技术，分析了水稻双苗品系SARII-628的单倍体与其相应二倍体在RNA水平上的基因表达情况。单倍体化后，在植株中观察到了不同程度的表达变化。主要结果如下：（1）单倍体化后，敏感位点的比例占芯片上设计的总位点的2.47%。这些位点随机分布在12对水稻染色体上，且激活的位点多于沉默的位点。（2）观察到33个序列在染色体上形成基因簇。（3）对575个敏感位点进行的GoPipe功能分类显示，这些位点参与了生物过程、细胞成分和分子功能。（4）RT-PCR总体上验证了微阵列的结果，符合率为83.78%。对于芯片分析中随机选择的激活或沉默位点，符合率高达91.86%。
