[应用三色荧光原位杂交技术对弱精子症患者精子X、Y及18号染色体的分析]

[An analysis on chromosome X, Y and 18 in the spermatozoa of asthenospermia patients by triple-color fluorescence in situ hybridization].

作者信息

Li Lian-Bing, Xia Yan-Kai, Li Xin-Sheng, Lü Jing, Ma Ming-Fu, Song Ling, Ji Yuan, Yang Ji-Gao, Zhang Tian-Feng, Chen Xiao-Xing, Rong Ying, Zhao Le-Tian

机构信息

Chongqing Institute of Science and Technology for National Population and Family Planning, Chongqing 400020, China.

出版信息

Zhonghua Nan Ke Xue. 2008 Mar;14(3):211-4.

PMID:18488331

Abstract

OBJECTIVE

To analyze the numerical aberration of chromosome X, Y and 18 in the spermatozoa of asthenospermia patients by triple-color fluorescence in situ hybridization.

METHODS

The experiment included 10 asthenospermia patients and 5 healthy men with normal semen quality as controls. Fluorescence in situ hybridization (FISH) and probes for chromosomes including X, Y and 18 were used to determine the frequency of the aneuploid of the chromosomes in spermatozoa.

RESULTS

Of the 45,547 spermatozoa counted from the semen samples, the hybridization rate was 99.18%. The frequencies of the chromosome disomies including XX18, XY18, YY18, X1818 and Y1818 were (0.124 +/- -0.086)%, (0.360 +/- 0.380)%, (0.109 +/- 0.195)%, (0.342 +/- 0.746)% and (0.299 +/- 0.564)% in the case group and (0.014 +/- 0.019)%, (0.090 +/- 0.080)%, (0.030 +/- 0.031)%, (0.068 +/- 0.103)% and (0.075 +/- 0.083)% in the control. The sperm aneuploid rate was 9.25% in the former and 2.70% in the latter, with significant difference in between (P< 0.01).

CONCLUSION

Asthenospermia patients have a higher aneuploid rate of sperm chromosome than normal fertile men. However, larger samples are yet to be studied to obtain more scientific evidence.

摘要

目的

通过三色荧光原位杂交技术分析弱精子症患者精子中X、Y和18号染色体的数目畸变情况。

方法

本实验纳入10例弱精子症患者，并选取5例精液质量正常的健康男性作为对照。采用荧光原位杂交（FISH）技术及X、Y和18号染色体探针，检测精子中染色体非整倍体的频率。

结果

在所计数的45547条精液样本精子中，杂交率为99.18%。病例组中XX18、XY18、YY18、X1818和Y1818染色体二体的频率分别为（0.124±0.086）%、（0.360±0.380）%、（0.109±0.195）%、（0.342±0.746）%和（0.299±0.564）%，对照组中分别为（0.014±0.019）%、（0.090±0.080）%、（0.030±0.031）%、（0.068±0.103）%和（0.075±0.083）%。前者精子非整倍体率为9.25%，后者为2.70%，两者差异有统计学意义（P<0.01）。