Li Lian-Bing, Xia Yan-Kai, Li Xin-Sheng, Lü Jing, Ma Ming-Fu, Song Ling, Ji Yuan, Yang Ji-Gao, Zhang Tian-Feng, Chen Xiao-Xing, Rong Ying, Zhao Le-Tian
Chongqing Institute of Science and Technology for National Population and Family Planning, Chongqing 400020, China.
Zhonghua Nan Ke Xue. 2008 Mar;14(3):211-4.
To analyze the numerical aberration of chromosome X, Y and 18 in the spermatozoa of asthenospermia patients by triple-color fluorescence in situ hybridization.
The experiment included 10 asthenospermia patients and 5 healthy men with normal semen quality as controls. Fluorescence in situ hybridization (FISH) and probes for chromosomes including X, Y and 18 were used to determine the frequency of the aneuploid of the chromosomes in spermatozoa.
Of the 45,547 spermatozoa counted from the semen samples, the hybridization rate was 99.18%. The frequencies of the chromosome disomies including XX18, XY18, YY18, X1818 and Y1818 were (0.124 +/- -0.086)%, (0.360 +/- 0.380)%, (0.109 +/- 0.195)%, (0.342 +/- 0.746)% and (0.299 +/- 0.564)% in the case group and (0.014 +/- 0.019)%, (0.090 +/- 0.080)%, (0.030 +/- 0.031)%, (0.068 +/- 0.103)% and (0.075 +/- 0.083)% in the control. The sperm aneuploid rate was 9.25% in the former and 2.70% in the latter, with significant difference in between (P< 0.01).
Asthenospermia patients have a higher aneuploid rate of sperm chromosome than normal fertile men. However, larger samples are yet to be studied to obtain more scientific evidence.
通过三色荧光原位杂交技术分析弱精子症患者精子中X、Y和18号染色体的数目畸变情况。
本实验纳入10例弱精子症患者，并选取5例精液质量正常的健康男性作为对照。采用荧光原位杂交（FISH）技术及X、Y和18号染色体探针，检测精子中染色体非整倍体的频率。
在所计数的45547条精液样本精子中，杂交率为99.18%。病例组中XX18、XY18、YY18、X1818和Y1818染色体二体的频率分别为（0.124±0.086）%、（0.360±0.380）%、（0.109±0.195）%、（0.342±0.746）%和（0.299±0.564）%，对照组中分别为（0.014±0.019）%、（0.090±0.080）%、（0.030±0.031）%、（0.068±0.103）%和（0.075±0.083）%。前者精子非整倍体率为9.25%，后者为2.70%，两者差异有统计学意义（P<0.01）。
弱精子症患者精子染色体非整倍体率高于正常生育男性。然而，仍需更大样本量的研究以获得更科学的证据。
